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Millipore/17-459 |磷酸-IRS1（Ser307小鼠/Ser312人）STAR ELISA试剂盒/17-459/96分析
Millipore/17-459 |磷酸-IRS1（Ser307小鼠/Ser312人）STAR ELISA试剂盒/17-459/96分析
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-459 |
| BrandFamily | Upstate |
| TradeName |
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| Description | phospho-IRS1(Ser307mouse/Ser312Human)STARELISAKit |
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| BackgroundInformation | I.TESTPRINCIPLE TheUPSTATE®colorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.TheIRS1plateiscoatedwithaspecificmousemonoclonalcaptureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingIRS1antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanyunboundnon-specificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-IRS1(Ser312)antibodytodetectthecapturedIRS1ontheplatewellthatisphosphorylatedonSer312.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader. Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve. II.IRS1BACKGROUND IRS(InsulinReceptorSubstrate)proteinsaretheeffectorsofbothInsulinandIGF-initiatedsignaling.Insulin,inadditiontoworkingverysimilarlytoagrowthfactorinstimulatingreceptortyrosinekinases,isessentialformaintainingglucosehomeostasisandregulatingcarbohydrate,lipid,andproteinmetabolism.Thesignalingeventsofinsulinaremediatedviaavastandhighlyintegratednetworkofmoleculesthatcontrolsseveralprocesses.Thereare4IRSproteins,1-4.Itisbelievedthattheseproteinsservecomplementaryfunctionsindifferenttissues.ThetwoprominentplayersareIRS1andIRS2withIRS1playingimportantrolesinskeletalmuscleandIRS2intheliver.TheIRSproteinslinktheinsulinreceptorwithmanysignalingpathwaysincludingthePI3Kinase/Akt/mTORandtheMAPKinasepathways.ThePI3K/Akt/mTORpathwayisinvolvedwithmanymetabolicactionsofinsulinsignaling.MAPKisinvolvedintheregulationofsomegenesandworkswithPI3Kinasetocontrolcellgrowthanddifferentiation.InadditiontotheinsulinreceptortyrosinekinaseactivityintypeIIdiabetespatientsandmice,studieshaveshownagreaterdefectincomponentsoftheinsulinsignalingpathwayincludingInsulinReceptorSubstrate(IRS)phosphorylationortheactivationofPhosphoinositide3-Kinase(PI3Kinase).Insulinstimulatesthesignalingcascadebybindingtotheinsulinreceptorthroughitsextracellularαsubunits.Thisstimulatesthetyrosinekinaseactivityoftheβsubunitsofthereceptor.ThereceptorthenautophosphorylatesitselfandphosphorylatestheIRS(InsulinReceptorSubstrate)proteinsthatareveryimportantmodulatorsofinsulinsignaling.IRSbindstothephosphorylatedtyrosinesoftheβsubunitoftheinsulinreceptor.TheysharePHandPTBdomainsneartheirN-termini,andmultipleTyrphosphorylationmotifsintheirC-terminalregions.Proteinswhichbindtotyrosine-phosphorylatedIRS-proteinsincludePI3Kinasep85,GRB2,SHP2,Nck,Crk,andFyn.IRS1appearstobeprincipallyinvolvedinIGF-signalingandcytoskeletalgrowth.IRS2appearstobethecriticalmediatorofInsulinsignaling,asgeneticablationresultsintypeIIdiabetes.IRS3isexpressedprimarilyinADIpocytesandisanunusuallypotentactivatorofPI3Kinase.IRS4lacksthetyrosineresidueswhichintheotherIRS-proteinsbindstoSHP2. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettorsandtipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater |
| ProductInformation | |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
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| StorageConditions | Maintaintheunopenedkitat2-8°Cuntilexpirationdate. Precautions •Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata. •CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial. •SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed. •TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin. •TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly. |
| Applications | |
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| Application | Thisphospho-IRS1(Ser307mouse/Ser312Human)STARELISAKitisusedtomeasure&quantifyIRS1levels. |
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| UniProtSummary | FUNCTION:SwissProt:P35568#Maymediatethecontrolofvariouscellularprocessesbyinsulin.WhenphosphorylatedbytheinsulinreceptorbindsspecificallytovariouscellularproteinscontainingSH2domainssuchasphosphatidylinositol3-kinasep85subunitorGRB2.Activatesphosphatidylinositol3-kinasewhenboundtotheregulatoryp85subunit(Bysimilarity). SIZE:1242aminoacids;131591Da SUBUNIT:InteractswiththeNPXYmotifoftyrosine-phosphorylatedIGF1RandINSRviathePTBdomain.Bindstophosphatidylinositol3-kinasep85subunitviathephosphorylatedYXXMmotifs.BindsROCK1.BindstoUBTFandPIK3CAinnuclearextracts(Bysimilarity).InteractswithSOCS7. PTM:SerinephosphorylationofIRS1isamechanismforinsulinresistance.Ser-312phosphorylationinhibitsinsulinactionthroughdisruptionofIRS1interactionwiththeinsulinreceptor(Bysimilarity).&PhosphorylationofTyr-896isrequiredforGRB2-binding(Bysimilarity).DISEASE:SwissProt:P35568#PolymorphismsinIRS1maybeinvolvedintheetiologyofnon-insulin-dependentdiabetesmellitus(NIDDM)[MIM:125853]. SIMILARITY:SwissProt:P35568##Contains1IRS-typePTBdomain.&Contains1PHdomain. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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