Millipore/MAB3776 |抗MDM2抗体，克隆SMP14/MAB3776/100µ；G
市场价：
¥6980.00
美元价：
4188.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB3776 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-MDM2Antibody,cloneSMP14 |
| AlternateNames |
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| BackgroundInformation | MDM2isover-expressedinmanytumors.Itsprincipalfunctionistheubiquitinationanddegradationofp53tumoursuppressorprotein.ThismonoclonalantibodyalsorecognizesapeptideepitopearoundThr-216ofmurineMDM2whenThr-216isunphosphorylated(Zhang&Prives,2001).SMP14alsocrossreactswithsomecytokeratins(6,14&16).Thisisonlyaproblemwhenworkingwithcertainepithelialcellsandnotfibroblasts. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Liquidin0.02Mphosphatebuffer,0.25MNaCl,pH7.6,with0.1%sodiumazide |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat2-8°Cinundilutedaliquotsforupto12monthsfromdateofreceipt. |
| Applications | |
|---|---|
| Application | UseAnti-MDM2Antibody,cloneSMP14(MouseMonoclonalAntibody)validatedinIP,IHC,IHC(P)todetectMDM2alsoknownasMurineDoubleMinute2. |
| KeyApplications |
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| ApplicationNotes | Westernblot Immunohistochemistry(FrozenandParaffin-embeddedsections) Immunoprecipitation ELISA Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Syntheticpeptidecorrespondingtoresidues154-167(CSRPSTSSRRRAISE)ofhumanMDM2cross-linkedtoKLHusingglutaldehyde. |
| Clone | SMP14 |
| Host | Mouse |
| Specificity | RecognizeshumanMDM2. |
| Isotype | IgG2a |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneisatargetgeneofthetranscriptionfactortumorproteinp53.Theencodedproteinisanuclearphosphoproteinthatbindsandinhibitstransactivationbytumorproteinp53,aspartofanautoregulatorynegativefeedbackloop.Overexpressionofthisgenecanresultinexcessiveinactivationoftumorproteinp53,diminishingitstumorsuppressorfunction.ThisproteinhasE3ubiquitinligaseactivity,whichtargetstumorproteinp53forproteasomaldegradation.Thisproteinalsoaffectsthecellcycle,apoptosis,andtumOrigenesisthroughinteractionswithotherproteins,includingretinoblastoma1andribosomalproteinL5.Morethan40differentalternativelysplicedtranscriptvariantshavebeenisolatedfrombothtumorandnormaltissues. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q00987#InhibitsTP53/p53-andTP73/p73-mediatedcellcyclearrestandapoptosisbybindingitstranscriptionalactivationdomain.FunctionsasaubiquitinligaseE3,inthepresenceofE1andE2,towardp53anditself.Permitsthenuclearexportofp53andtargetsitforproteasome-mediatedproteolysis. SIZE:491aminoacids;55233Da SUBUNIT:Bindsp53,p73,ARF(P14),ribosomalproteinL5andspecificallytoRNA.Caninteractalsowithretinoblastomaprotein(RB),E1A-associatedproteinEP300andtheE2F1transcriptionfactor.Formsaternarycomplexwithp53andWWOX.InteractswithUSP7andTBRG1.IsoformMdm2-FdoesnotinteractwithTP53/p53.Interactswith,andubiquitinatesHIV-1Tat. SUBCELLULARLOCATION:Nucleus,nucleoplasm.Cytoplasm.Nucleus,nucleolus.Note=Expressedpredominantlyinthenucleoplasm.InteractionwithARF(P14)resultsinthelocalizationofbothproteinstothenucleolus.ThenucleolarlocalizationsignalsinbothARF(P14)andMDM2maybenecessarytoallowefficientnucleolarlocalizationofbothproteins. TISSUESPECIFICITY:Ubiquitous.IsoformMdm2-A,isoformMdm2-B,isoformMdm2-C,isoformMdm2-D,isoformMdm2-E,isoformMdm2-FandisoformMdm2-Gareobservedinarangeofcancersbutabsentinnormaltissues. DOMAIN:SwissProt:Q00987RegionIissufficientforbindingp53andinhibitingitsG1arrestandapoptosisfunctions.Italsobindsp73andE2F1.RegionIIcontainsmostofacentralacidicregionrequiredforinteractionwithribosomalproteinL5andaputativeC4-typezincfinger.TheRINGfingerdomainwhichcoordinatestwomoleculesofzincinteractsspecificallywithRNAwhetherornotzincispresentandmediatesthehetero-oligomerizationwithMDM4.ItisalsoessentialforitsubiquitinligaseE3activitytowardp53anditself. PTM:PhosphorylatedinresponsetoionizingrADIationinanATM-dependentmanner. DISEASE:SwissProt:Q00987#Seemstobeamplifiedincertaintumors(includingsofttissuesarcomas,osteosarcomasandgliomas).Ahigherfrequencyofsplicevariantslackingp53bindingdomainsequenceswasfoundinlate-stageandhigh-gradeovarianandbladdercarcinomas.Fourofthesplicevariantsshowlossofp53binding. SIMILARITY:BelongstotheMDM2/MDM4family.&Contains1RanBP2-typezincfinger.&Contains1RING-typezincfinger.&Contains1SWIBdomain. MISCELLANEOUS:MDM2RINGfingermutationsthatfailedtoubiquitinatep53invitrodidnottargetp53fordegradationwhenexpressedincells. |
| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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