Millipore/07-575 | Anti-MDM2 Antibody/07-575/200 µg
市场价：
¥6160.00
美元价：
3696.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-575 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-MDM2Antibody |
| Overview | Anti-MDM2 |
| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | 0.1MTris-glycine,pH7.4,0.15MNaCl,0.05%sodiumazidebeforetheadditionofglycerolto30% |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 2yearsat-20°C |
| Applications | |
|---|---|
| Application | DetectMDM2alsoknownasMurineDoubleMinute2withAnti-MDM2Antibody(RabbitPolyclonalAntibody),thathasbeendemonstratedtoworkinWB. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| Immunogen | peptide(C-AISETEENSDELSGERQRKRHK)correspondingtoaminoacids164-185ofhumanMDM2. |
| Host | Rabbit |
| Specificity | MDM2 |
| Isotype | IgG |
| SpeciesReactivity |
|
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneisatargetgeneofthetranscriptionfactortumorproteinp53.Theencodedproteinisanuclearphosphoproteinthatbindsandinhibitstransactivationbytumorproteinp53,aspartofanautoregulatorynegativefeedbackloop.Overexpressionofthisgenecanresultinexcessiveinactivationoftumorproteinp53,diminishingitstumorsuppressorfunction.ThisproteinhasE3ubiquitinligaseactivity,whichtargetstumorproteinp53forproteasomaldegradation.Thisproteinalsoaffectsthecellcycle,apoptosis,andtumOrigenesisthroughinteractionswithotherproteins,includingretinoblastoma1andribosomalproteinL5.Morethan40differentalternativelysplicedtranscriptvariantshavebeenisolatedfrombothtumorandnormaltissues. |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q00987#InhibitsTP53/p53-andTP73/p73-mediatedcellcyclearrestandapoptosisbybindingitstranscriptionalactivationdomain.FunctionsasaubiquitinligaseE3,inthepresenceofE1andE2,towardp53anditself.Permitsthenuclearexportofp53andtargetsitforproteasome-mediatedproteolysis. SIZE:491aminoacids;55233Da SUBUNIT:Bindsp53,p73,ARF(P14),ribosomalproteinL5andspecificallytoRNA.Caninteractalsowithretinoblastomaprotein(RB),E1A-associatedproteinEP300andtheE2F1transcriptionfactor.Formsaternarycomplexwithp53andWWOX.InteractswithUSP7andTBRG1.IsoformMdm2-FdoesnotinteractwithTP53/p53.Interactswith,andubiquitinatesHIV-1Tat. SUBCELLULARLOCATION:Nucleus,nucleoplasm.Cytoplasm.Nucleus,nucleolus.Note=Expressedpredominantlyinthenucleoplasm.InteractionwithARF(P14)resultsinthelocalizationofbothproteinstothenucleolus.ThenucleolarlocalizationsignalsinbothARF(P14)andMDM2maybenecessarytoallowefficientnucleolarlocalizationofbothproteins. TISSUESPECIFICITY:Ubiquitous.IsoformMdm2-A,isoformMdm2-B,isoformMdm2-C,isoformMdm2-D,isoformMdm2-E,isoformMdm2-FandisoformMdm2-Gareobservedinarangeofcancersbutabsentinnormaltissues. DOMAIN:SwissProt:Q00987RegionIissufficientforbindingp53andinhibitingitsG1arrestandapoptosisfunctions.Italsobindsp73andE2F1.RegionIIcontainsmostofacentralacidicregionrequiredforinteractionwithribosomalproteinL5andaputativeC4-typezincfinger.TheRINGfingerdomainwhichcoordinatestwomoleculesofzincinteractsspecificallywithRNAwhetherornotzincispresentandmediatesthehetero-oligomerizationwithMDM4.ItisalsoessentialforitsubiquitinligaseE3activitytowardp53anditself. PTM:PhosphorylatedinresponsetoionizingrADIationinanATM-dependentmanner. DISEASE:SwissProt:Q00987#Seemstobeamplifiedincertaintumors(includingsofttissuesarcomas,osteosarcomasandgliomas).Ahigherfrequencyofsplicevariantslackingp53bindingdomainsequenceswasfoundinlate-stageandhigh-gradeovarianandbladdercarcinomas.Fourofthesplicevariantsshowlossofp53binding. SIMILARITY:BelongstotheMDM2/MDM4family.&Contains1RanBP2-typezincfinger.&Contains1RING-typezincfinger.&Contains1SWIBdomain. MISCELLANEOUS:MDM2RINGfingermutationsthatfailedtoubiquitinatep53invitrodidnottargetp53fordegradationwhenexpressedincells. |
| MolecularWeight | 76kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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