密理博/AG325 |氟翡翠®C/AG325/50毫克
市场价：
¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | AG325 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Fluoro-Jade®C |
| Overview | Fluoro-Jade®C,likeitspredecessors,Fluoro-Jade®andFluoro-Jade®B,werefoundtostainalldegeneratingneurons,regardlessofspecificinsultormechanismofcelldeath.Therefore,thepatternsofneuronaldegenerationseenfollowingexposuretoeithertheglutamateagoNIST,kainicacid,ortheinhibitorofmitochondrialrespiration,3-NPA,werethesameforalloftheFluoro-Jadeâdyes.However,therewasaqualitativedifferenceinthestainingcharacteristicsofthethreeFluorochromes.Specifically,Fluoro-Jade®Cexhibitedthegreatestsignaltobackgroundratio,aswellasthehighestresolution.Thistranslatestoastainofmaximalcontrastandaffinityfordegeneratingneurons.Thismakesitidealforlocalizingnotonlydegeneratingnervecellbodies,butalsodistaldendrites,axonsandterminals.ThedyeishighlyresistanttofADIngandiscompatIBLewithvirtuallyallhistologicalprocessingandstainingprotocols.TriplelabelingcanbeaccomplishedbystainingdegeneratingneuronswithFluoro-Jade®C,cellnucleiwithDAPIandactivatedastrocyteswithGFAPimmunofluorescence. APPEARANCE:Coffeebrowntobrickredpowder. MOLECULARWEIGHT:823 EXCITATIONPEAK:485nm EMISSIONPEAK:525nm FILTERSYSTEM:Fluorescein/FITC SOLUBILITY:Highlysolubleinwaterandbases,moderatelysolubleinalcoholandweakacids. TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption. |
| BackgroundInformation | Fluoro-JadeC,likeitspredecessors,Fluoro-JadeandFluoro-JadeB,werefoundtostainalldegeneratingneurons,regardlessofspecificinsultormechanismofcelldeath.Therefore,thepatternsofneuronaldegenerationseenfollowingexposuretoeithertheglutamateagonist,kainicacid,ortheinhibitorofmitochondrialrespiration,3-NPA,werethesameforalloftheFluoro-Jadedyes.However,therewasaqualitativedifferenceinthestainingcharacteristicsofthethreefluorochromes.Specifically,Fluoro-JadeCexhibitedthegreatestsignaltobackgroundratio,aswellasthehighestresolution.Thistranslatestoastainofmaximalcontrastandaffinityfordegeneratingneurons.Thismakesitidealforlocalizingnotonlydegeneratingnervecellbodies,butalsodistaldendrites,axonsandterminals.Thedyeishighlyresistanttofadingandiscompatiblewithvirtuallyallhistologicalprocessingandstainingprotocols.TriplelabelingcanbeaccomplishedbystainingdegeneratingneuronswithFluoro-JadeC,cellnucleiwithDAPIandactivatedastrocyteswithGFAPimmunofluorescence.Fluoro-Jade™isaregisteredtrademarkofHisto-Chem,Inc. |
| ProductInformation |
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| StorageandShippingInformation | |
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| StorageConditions | Thelyophilizedpowdershouldbestoredwellsealedatroomtemperature,preferableinadesiccator(duetoitshygroscopicnature)foruptooneyearfromdateofreceipt.Theliquidstocksolution(0.01%)indistilledwatercanbestoredat2-8°Cforupto3months.The.0002-.0001%workingsolutionin0.1%aceticacidshouldbeusedwithin4hrsofpreparation. |
| Applications | |
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| KeyApplications |
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| ApplicationNotes | SUGGESTEDPROTOCOLFORUSINGFLUORO-JADE®C Processing:Halfofeachgroupofbrainswereparaffinembeddedandcutonarotarymicrotomewhiletheremainderwerecutonafreezingslidingmicrotome.Paraffinsectionswere10uminthicknesswhilefrozensectionswerecutatathicknessof25um.Priortostaining,sectionsweremountedfromdistilledwaterontogelledslides.Gelatincoatedslideswerepreparedbyimmersionina60degreeCsolutionof1%pigskingelatin(Sigma;typeA,300Bloom)andthenovendriedovernightatthesametemperature.Thesectionsweremountedontotheslidesfromdistilledwaterandthenairdriedforatleast30minonaslidewarmerat50degreesC.Slidesbearingfrozencuttissuesectionswerefirstimmersedinabasicalcoholsolutionconsistingof1%sodiumhydroxidein80%ethanolfor5min.Theywerethenrinsedfor2minin70%ethanol,for2minindistilledwater,andthenincubatedin0.06%potassiumpermanganatesolutionfor10min.Followinga1-2minwaterrinse,theslideswerethentransferredfor10mintoa0.0001%solutionofFluoro-Jade®Cdissolvedin0.1%aceticacidvehicle.Theproperdilutionwasaccomplishedbyfirstmakinga0.01%stocksolutionofthedyeindistilledwaterandthenadding1mLofthestocksolutionto99mLof0.1%aceticacidvehicle.Theworkingsolutionwasusedwithin2hofpreparation.Thestocksolution,whenrefrigerated,canbekeptforlongperiodsbutshouldbediscardedifthesolutionbecomescloudy.Theslideswerethenrinsedthroughthreechangesofdistilledwaterfor1minperchange.Excesswaterwasdrainedontoapapertowel,andtheslideswerethenairdriedonaslidewarmerat50degreesCforatleast5min.Theairdriedslideswerethenclearedinxyleneforatleast1minandthencoverslippedwithDPX(FlukaorSigma)nonfluorescentmountingmedia.Polarcoverslippingmedia,suchasthosethatcontainwater,alcoholorglycerolwereneverused.Forcomparativepurposes,someslideswerestainedwithFluoro-Jade®Baccordingtothepreviouslydescribedprocedure.Whenworkingwithparaffinprocessedtissue,thesectionsarefirstdeparaffinizedthroughtwo10minchangesofxyleneandthenthesectionsarerehydratedthroughagraduatedalcoholseries,omittingthebasicalcoholsolution.Onceindistilledwater,thesectionsaretransferredtothepotassiumpermanganatesolutionatwhichpointthestainingprocedureisidenticaltothatdescribedforfrozensections.Multiplelabeling:Fluoro-Jade®CcanreadilybecombinedwithotherfluorescentMarkers.Multiplelabelingwasachievedusinganti-glialfibrillaryacidicprotein(GFAP)immunocytochemistrytolabelactivatedastrocyteswhileusingDAPItolabelnuclearDNA.Incorporating4",6-diamidino-2-phenylindole(DAPI;Sigma,St.LouisMO)asafluorescentnuclearstainisaccomplishedbysimplyincorporating0.0001%intotheFluoro-Jade®Cstainingsolution.Thisisaccomplishedbytheadditionof1mlof0.01%DAPIstocksolutionto99mlof0.1%aceticacid.Fluoro-Jade®CwasalsocombinedwithimmunofluorescentlabelingofGFAPaccordingtothefollowingprocedure.Loosefrozentissuesectionswereincubatedinapredilutedsolutionofanti-GFAP(ChemiconhasanumberofdifferentantibodiestoGFAP)atabout5degreesCintherefrigeratorfor1-3days.Itshouldbementionedthatalthoughinthisstudyallimmunocytochemistrywasperformedonfrozensections,themethodsarefullycompatiblewithparaffinprocessedtissueaswell.Sectionswererinsedintwochangesofbufferedsalinefor10mineachandthentransferredtoatetramethylrhodamineisothiocynate(TRITC)labeledsecondaryantibody(ChemiconhasanumberofdifferentTRITClabeledsecondaryantibodies),diluted1:100inbufferedsaline,for1hatroomtemperature.Sectionswererinsedintwochangesofbufferedsalinefor10mineachandthenthesectionsweremountedontogelledslidesfromdistilledwaterandairdriedonaslidewarmerat50degreesCfor30min.TocombinewithFluoro-Jade®C,theslidemountedsectionswererehydratedfor2minindistilledwaterandthentransferredtothe0.06%potassiumpermanganatesolutionfor10min.Itisworthmentioningthattheincubationtimeinpotassiumpermanganatemayneedtobereducedwhenco-localizingthoseantigenicepitopessusceptibletochemicaloxidation.Theslideswerethenrinsedfor2minindistilledwater,transferredtotheFluoro-Jade®Cworkingsolutionfor10minandthenrinsed,airdehydrated,xyleneclearedandcoverslippedwithDPX,aspreviouslydescribed.ThebluenuclearlabelconferredbyDAPIisvisualizedviaultravioletlightexcitation,whiletheredTRITClabeledantibodyisvisualizedbygreenlightexcitation. |
| BIOLOGicalInformation | |
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| Purity | SilicaTLC(acetanitrile/water,6/4)revealedthepresenceoftwofluorescentspots,presumablycorrespondingtothedisulphatehomologues.Thepresenceofprecursorsorfreefluoresceinwasnotdetected. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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