密理博/NS830 | GFAP ELISA试剂盒/NS830/1 kit96孔
市场价：
¥10480.00
美元价：
6288.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | NS830 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | GFAPELISAKit |
| Overview | Background GlialFibrillaryAcidicProtein(GFAP)isaclass-IIIintermediatefilamentuniquelyandubiquitouslyexpressedaspartofthestructuralnetworkinastrocyticglialcells.ThisspecificexpressionmakesGFAPidealasabroadastrocyticMarkerinvertebrates.Astrocytesplayavarietyofkeyrolesinsupporting,guiding,nurturing,andsignalingneuronalarchitectureandactivityinahighlyinterdependentmanner.CurrentunderstandingofCNSstructuresuggeststhatthereareatleastasmanyglialcellsastheestimated100billionneurons,butperhapsupwardsof100timesmore.Thus, thehealthofbothneuronsandastrocytesisoftenaffectedindiseaseandtrauma.GFAPisprovingtobeausefulbiomarkerinthedetectionofneuraldegeneration.AlthoughdefectsinGFAPhavebeenreportedtocausecertainrareconditions,suchasAlexander’sdisease(Brenneretal.,2001),theobservedinterconnectedphysiologyofneuronalandglialcellshasenabledtheuseofGFAPasaneurodegenerationdetectiongauge.Thisrelationshipisbeingexploitedbyresearchersandcliniciansmeasuringavarietyofneurologicalissuesfromcancertotrauma.GFAPhasbeenusedasabiomarkerintheearlydetectionofchemicallyinducedcancer(Capoetal.,1997)andclassicalglioblastomas(Jungetal.,2007).ElevatedlevelsofGFAPhavebeenreportedintraumaticbraininjury(TBI)andappeartodirectlycorrelatewiththeseverityofinjuryandbraintissueoxygenationincombatcasualtycareresearch(CCR,2008)aswellascivilianaccidents(Mondelloetal.,2010).GFAPhasalsobeenidentifiedasagoodbiomarkerinstudiesoftoxininducedacutebraininjury(Liaoetal.,2008),indifferentiationofhemorrhagicvs.ischemicstrokes(Foerchetal.,2006),aswellasincardiacarrest(Kanekoetal.,2009).MilliporenowoffersanELISAassayforsensitivemeasurementofGFAPproteinintissuehomogenatesandBIOLOGicalfluids(serum,plasma,CSF,andserumfreemedia)fromhuman,mouse,andrat. PrincipalsofProcedure ThisassayisaSandwichELISAbasedsequentiallyon:1)captureofsolubleGFAPproteintothewellsofamicrotiterplatecoatedbyapre-titeredamountofanti-GFAPmonoclonalantibody;2)removalofunboundmaterialbywashing;3)bindingofbiotinylatedanti-GFAPmonoclonalantibodyto thepreviouslycapturedGFAPprotein;4)removalofunboundmaterialbywashing;5)bindingofstreptavidinconjugatedhorserADIshperoxidasetothebiotinylatedantibodies;6)removalofexcessorunboundenzymeconjugatesbywashing;and7)quantificationofimmobilizedantibody-enzyme complexesbymonitoringhorseradishperoxidaseactivityinthepresenceofthesubstrate3,3’,5,5’-tetramethylbenzidine(TMB).Enzymeactivityismeasuredspectrophotometricallybyrecordingabsorbancereadingsat450nm.Increasesinabsorbencyaredirectlyproportionaltotheamountof GFAPproteincapturedfromthesample.ThereforetheproteinconcentrationofunknownscanbederivedbyinterpolationusingastandardcurvegeneratedinthesameassaywithreferencestandardsofknownconcentrationsofGFAP. |
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| MaterialsRequiredbutNotDelivered | 1.Absorbentpaperorcloth 2.Conventional,multi-channel,orrepeatingPipettes 3.Reagentreservoirs 4.Orbitalmicrotiterplateshaker/washer(optional) 5.GraduatedSEROlogicalpipettes 6.Microtiterplatereadercapableofreadingabsorbencyat450nm 7.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 8.Microfugetubesforstandardandsampledilutions 9.Mechanicalvortex 10.Milli-Q™Water |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storage:TheGFAPELISAkitisshippedoniceandshouldbestoredat2-8°Cuponarrival. StABIlity:Kitcomponentsarestableforaminimumof6monthsfromthedateofreceiptifstoredand handledasdescribedabove. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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