Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit
市场价：
¥12600.00
美元价：
7560.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ECM340 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | HumansVCAM-1ELISA |
| Overview | Vascularcelladhesionmolecule-1(VCAM-1,CD106)isamemberoftheimmunoglobulingenesuperfamily.TwoformsofVCAM-1withsixorsevenextracellularIg-likedomainsariseduetoalternativesplicingfromaseven-domainVCAM-1(7DVCAM-1).7DVCAM-1isthedominantformexpressedbyculturedhumanendothelialcells[Cybulskyetal.,1991;Hessionetal.,1991].Homologyofdomains1through3withdomains4through6suggestsageneduplicationevent[Cybulskyetal.,1991;Hessionetal.,1991].TheCDNAof7DVCAM-1predictsacoreproteinofapproximately81kDawithsevensitesforN-linkedglycosylation.Uponcompleteglycosylationthematureproteinhasamolecularweightofapproximately102kDa.Thisobservationisingeneralagreementwiththe110kDaproteinimmunoprecipitatedfromcytokine-activatedendothelium[Carlosetal.,1990;Riceetal.,1990]. VCAM-1appearstohavebeenhighlyconservedthroughevolution.BothratandmouseVCAM-1arehighlyhomologousattheproteinleveltothehumanVCAM-1(77%and76%,respectively)[Hessionetal.,1992]. VCAM-1supportstheadhesionoflymphocytes,monocytes,naturalkillercells,eosinophilsandbasophils,throughitsinteractionwithintegrina4b1(VLA-4).VCAM-1/VLA-4interactionmediatesfirmadherenceofcirculatingnon-neutrophilicleukocytestoendothelium[Shararetal.,1995].VCAM-1alsoparticipatesinleukocyteadhesionoutsideofthevasculature,mediatingprecursorlymphocyteadhesiontobonemarrowstromalcells[Ryanetal.1991]andBcellbindingtolymphnodefolliculardendriticcells[Feedmanetal.,1992].VCAM-1isnotconstitutivelyexpressedonendothelium,butcanbeup-regulatedinvitroinresponsetoLPS,TNF-a,andIL-1[Carlos&Harlan,1990;Osbornetal.,1989],aswellastointerferon-gandIL-4[Masinovskyetal.1990].VCAM-1isalsopresentontissuemacrophages,dendritecells,bonemarrowfibroblasts,myoblastsandmyotubes. AsolubleformofVCAM-1hasbeendescribed[DeMaeyer&DeMaeyer-Buignard,1995;Lobbetal.,1991],andsolubleVCAM-1hasbeenfoundinserum[Gearingetal.,1995].IncreasedlevelsofsolubleVCAM-1canbedetectedinseveraldiseasestates,includingcancer[Banksetal.,1993;Fortisetal.,1995;Gearing&Newman,1993;Laietal.,1995],autoimmunedisorders[Gearing&Newman,1993;Doreduffyetal.,1995;Gruschwitzetal.,1995;Masonetal.,1993;Mrowka&Sieberth,1995;Spronketal.,1994;Wellicomeetal.,1993],infections[Jakobsenetal.,1994]andinflammations[Gearing&Newman,1993;Mrowka&Sieberth,1995;Adamsetal.,1995;Limetal.,1995]. TestPrinciple: Ananti-VCAM-1monoclonalantibodyisadsorbedontomicrowells. SolubleVCAM-1presentinasampleorstandardthenbindstoantibodiesadsorbedtothemicrowells.Amixtureofbiotin-conjugatedmonoclonalanti-VCAM-1antibodyandStreptavidin-HRPconjugateisadded.Biotinylatedanti-VCAM-1antibodybindstoVCAM-1capturedbythefirstantibody. Streptavidin-HRPbindstothebiotinylatedanti-VCAM-1.Unboundbiotinylatedanti-VCAM-1andStreptavidin-HRPconjugateisremovedwithawashstepandHRPsubstratesolutionisaddedtothewells. ThepairofmonoclonalantibodiesusedinthisELISAdetectthesolubleformofVCAM-1presentinserum,plasma,urine,andotherBIOLOGicalfluids. Anamountofcoloredproductisformed,proportionaltotheamountofsolubleVCAM-1presentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromsixsolubleVCAM-1standarddilutionsandtheVCAM-1sampleconcentrationisdetermined. Application: TheVCAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleVascularCellAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherbiologicalfluids.TheVCAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures. |
| AlternateNames |
|
| MaterialsRequiredbutNotDelivered | -5mLand10mLgraduatedPipettes -5μLto1000μLadjustablesinglechannelmicropipetteswith disposabletips -50μLto300μLadjustablemultichannelmicropipettewithdisposabletips -Multichannelmicropipettereservoir -Beakers,flasks,cylindersnecessaryforpreparationofreagents -Devicefordeliveryofwashsolution(multichannelwashbottleor automaticwashsystem) -MicrowellstripreadercapableofreADIngat450nm(620nmas optionalreferencewavelength) -Glass-distilledordeionizedwater -Statisticalcalculatorwithprogramtoperformlinearregressionanalysis. |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Precautions: ·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures. ·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources. ·Donotusekitreagentsbeyondexpirationdateonlabel. ·Donotexposekitreagentstostronglightduringstorageorincubation. ·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens. ·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly. ·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal. ·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes. ·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents. ·Exposuretoacidswillinactivatetheconjugate. ·Glass-distilledwaterordeionizedwatermustbeusedforreagent preparation. ·Substratesolutionsmustbeatroomtemperaturepriortouse. ·Sinceexactconditionsmayvaryfromassaytoassay,astandard curvemustbeestablishedforeveryrun. ·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults. ·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse. ·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods. ·Anyhumananti-mouseIgGantibody(HAMA)presentmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent. |
| Applications | |
|---|---|
| Application | ThisHumansVCAM-1ELISAisusedtomeasure&quantifysVCAM-1levelsinInflammation&Immunology&Neuroscienceresearch. |
| KeyApplications |
|
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
