微孔/ECM335 |人sICAM-1 ELISA/ECM335/1试剂盒
市场价：
¥12600.00
美元价：
7560.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | ECM335 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | HumansICAM-1ELISA |
| Overview | IntercellularAdhesionMolecule-1(ICAM-1)isamemberoftheimmunoglobulinsupergenefamily[Rothleinetal.,1988]andfunctionsasaligandforintegrinalphaLbeta2orLymphocyteFunction-AssociatedAntigen-1(LFA-1),amemberoftheleukocyteintegrinfamily[Marin&Springer1987]whichmediateslymphocyteadhesion. ICAM-1isasingle-chainglycoproteinwithapolypeptidecoreof55kDaexpressedonnon-hematopoieticcellsofmanylineagesincludingvascularendothelialcells,thymicepithelialcells,otherepithelialcellsandfibroblasts,andonhematopoieticcellssuchastissuemacrophages,mitogen-stimulatedT-lymphoblasts,germinalcenterB-cellsandthedendriticcellsfromtonsils,lymphnodesandPeyer"spatches.ICAM-1isinducIBLeonfibroblastsandendothelialcellsbytheinflammatorymediatorsIL-1,TNFandIFN-gamma.Itspresencecorrelateswithinfiltrationoflymphocytesintoinflammatorylesions[Dustinetal.,1989;Proberetal.,1986;Rothleinetal.,1986].ICAM-1seemstobetheinitialMarkerofinflammatoryreactionsandisexpressedpriortoandtoagreaterextentthanHLA-DR. TheroleofICAM-1asadiseasemarkerhasbeendemonstratedforanumberofdifferentpathologies,includingallergicrhinitis,allergiccontactdermatitis[Vejlsgaardetal.1989],gastrointestinalandbladdercancer,lymphoproliferativedisorders[Laletal.,1992;Rothleinetal.,1991],melanoma[Altomoneetal.,1992;Beckeretal.,1992;harningetal.,1991],HIV-1infection[Mostetal.,1993],malaria,tissueororgantransplantrejection[Adamsetal.,1989;Adamsetal.,1993],diabetesmellitus[Lampeteretal.,1992],glomerulonephritis,asthma[Wegneretal.,1990],rheumatoidarthritis[Popocniketal.,1990]andpsoriasis[Lisbyetal.,1989]. Apanelof50serafromhealthyblooddonors(maleandfemale)wastestedforsICAM-1.ThedetectedsICAMlevelsrangedbetween129.9and297.4ng/mlwithameanof230.3ng/mlandastandarddeviationof47.4ng/ml.NormalsICAM-1levelsmayvarydependingontheserumcollectiveusedrangingupto400ng/mlorgreater.Dependinguponthestudypopulationrangesfrom219ng/mlto1050ng/mlhavebeenreportedintheliterature. AnalyticalSensitivityandDetectionLimits: Sensitivity:3.3ng/mL RangeofDetection:6.25-100ng/mL Intra-AssayVariation:4.1% Inter-AssayVariation:7.7% Recovery:98.6%average AssayTime:75minutes Crossreactivity:nointerferencefromsolubleTNF-R(60or80kDa),IL-8/NAP-1,TNF-alpha,TNF-beta,IFN-gamma,IFN-alpha2C,IFN-w,IL-6,IL-2R,E-selectin-1orL-selectin-1. TheICAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleIntercellularAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherBIOLOGicalfluids.TheICAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures. TestPrinciple: Ananti-ICAM-1monoclonalantibodyisadsorbedontomicrowells.ThepairofmonoclonalantibodiesuseinthisELISAassaydetectthesoluableformofICAM-1presentinserum,plasmaorotherbiologicalfluids. SolubleICAM-1presentinasampleorstandardthenbindstoantibodiesadsorbedtothemicrowells.Asecond,HRP-conjugatedmonoclonalanti-ICAM-1antibodyisaddedandbindstoICAM-1capturedbythefirstantibody. Unboundenzyme-conjugatedanti-ICAM-1isremovedwithawashstepandHRPsubstratesolutionisaddedtothewells. Anamountofcoloredproductisformed,proportionaltotheamountofsolubleICAM-1presentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromfiveICAM-1standarddilutionsandtheICAM-1sampleconcentrationisdetermined. Application: TheICAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleIntercellularAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherbiologicalfluids.TheICAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures. |
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| MaterialsRequiredbutNotDelivered | -5mLand10mLgraduatedPipettes -5μLto1000μLadjustablesinglechannelmicropipetteswith disposabletips -50μLto300μLadjustablemultichannelmicropipettewithdisposabletips -Multichannelmicropipettereservoir -Beakers,flasks,cylindersnecessaryforpreparationofreagents -Devicefordeliveryofwashsolution(multichannelwashbottleor automaticwashsystem) -MicrowellstripreadercapableofreADIngat450nm(620nmas optionalreferencewavelength) -Glass-distilledordeionizedwater -Statisticalcalculatorwithprogramtoperformlinearregression analysis. |
| ProductInformation | |
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| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
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| StorageConditions | Storekitreagents,exceptthecontrol,between2°and8°C.Storethelyophilizedcontrolat-20°C.Immediatelyafteruseremainingreagentsshouldbereturnedtocoldstorage(2°to8°C)withtheexceptionofreconstitutedcontrol(-20°C).Expirationdateofthekitisstatedonoutsidelabel. Precautions: ·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures. ·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources. ·Donotusekitreagentsbeyondexpirationdateonlabel. ·Donotexposekitreagentstostronglightduringstorageor incubation. ·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens. ·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.Thimerosalisapossiblemutagenandshouldbehandledaccordingly. ·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal. ·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes. ·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents. ·Exposuretoacidswillinactivatetheconjugate. ·Glass-distilledwaterordeionizedwatermustbeusedforreagent preparation. ·Substratesolutionsmustbeatroomtemperaturepriortouse. ·Sinceexactconditionsmayvaryfromassaytoassay,astandard curvemustbeestablishedforeveryrun. ·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults. ·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse. ·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods. ·Theuseofradioimmunotherapyhassignificantlyincreasedthenumberofpatientswithhumananti-mouseIgGantibody(HAMA).HAMAmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent. |
| Applications | |
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| Application | ThisHumansICAM-1ELISAisusedtomeasure&quantifysICAM-1levelsinInflammation&Immunology&Neuroscienceresearch. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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