微孔/CYT420 |化学因子™ 色素上皮衍生因子夹心ELISA/CYT420/1试剂盒
市场价：
¥8740.00
美元价：
5244.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | CYT420 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | ChemiKine™PigmentEpitheliumDerivedFactor,SandwichELISA |
| Overview | Introduction PigmentEpithelium-DerivedFactor(PEDF)wasfirstidentifiedasafactorsecretedbyprimaryretinalpigmentepithelial(RPE)cellsthatpromotesthesurvivalofneuronsinvitroandinvivo.MolecularcharacterizationofPEDFshowsittobea50kDamemberoftheserpinfamilyofproteaseinhibitors1.AsecondmodeofactionofPEDFwasidentifiedwiththedemonstrationthatPEDFinducesapoptosisofmicrovascularendothelialcells,apropertythatmaintainstheavascularnatureoftheretina2.Asanumberofophthalmologicdisordersarecharacterizedbydysregulationoftheretinalinnervationandmicrovasculature,PEDFservesasanimportantregulatorofhumaneyedisease3.Inaddition,PEDFappearstohavemultifunctionalantitumoractivityinaneuroblastomamodel,asSchwanncell-derivedPEDFinducesadifferentiated,lessmalignantphenotypeinneuroblastomacells,promotesfurthergrowthandsurvivalofSchwanncells,andinhibitsangiogenesis4. ChemiKine™PigmentEpithelium-DerivedFactor(PEDF)kitisasandwichstripwell-typeenzymeimmunoassay(EIA),whichmeasuresPEDF.ThekitwillmeasurePEDFofvitreousfluidofhumanorigin. TestPrinciple WiththeChemiKine™PEDFassaysystem,amousemonoclonalantibodygeneratedagainsthumanPEDFiscoatedontoamicroplateandisusedtocapturePEDFfromasample.Asecond,biotinylatedPEDF-specificmousemonoclonalantibodydetectsthecapturedPEDF.Peroxidase-labeledstreptavidindetectsthebiotinylatedmousemonoclonalantibody.AfteradditionofthesubstrateandstopsolutiontheamountofPEDFisdetermined.ThestandardcurvedemonstratesadirectrelationshipbetweenOpticalDensity(OD)andPEDFconcentration:i.e.,thehighertheODthehigherthePEDFconcentrationinthesample. |
| AlternateNames |
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| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.37ºCincubator 3.Platesealers 4.Pipettors&tipscapableofaccuratelymeasuring10-1000mL 5.GraduatedSEROlogicalpipettes 6.96-wellmicroplatereaderwith450nmfilter 7.Graphpaperformanualplottingofdata 8.Polypropylenetesttubesforstandardandsampledilutions 9.Mechanicalvortex 10.One1or2litercontainer 11.Urea |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Colorimetric |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintaintheunopenedkitat2-8°Cuntilexpirationdateindicatedonthelabel.Afteropeningthekitmaintaineachcomponentat2-8°Cuntilexpirationdateindicatedonthelabel.Afterreconstitution,maintainthePEDFStandardat-20ºCforupto30days.Thestandardmaybefrozenandthawedthreetimeswithoutlossofimmunoreactivity.Donotstorereconstitutedstandardat4ºC,aslossofimmunoreactivitywillresult. Precautions ·WashBufferandAssayDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly. ·Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata. |
| Applications | |
|---|---|
| Application | ThisChemiKinePigmentEpitheliumDerivedFactor,SandwichELISAisusedtomeasure&quantifyPEDFlevelsinNeuroscienceresearch. |
| KeyApplications |
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| ApplicationNotes | ChemiKine™PEDFkitisdesignedtomeasuretheamountofPEDFincellculturesupernatants,tissuehomogenatesandBIOLOGicalfluid(vitreous,serum,plasma,andserum-free)samplesofhumanorigin.Thereareenoughreagentsincludedinthiskitforone96-wellimmunoassayplate.Runningduplicatewellsforsamplesandstandardsisrecommended. ForResearchUseOnly.Notforuseindiagnosticprocedures. |
| PhysicochemicalInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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