微孔/NS170 |磷酸化神经丝（pNF-H）夹心ELISA试剂盒/NS170/1 kit96孔
市场价：
¥11620.00
美元价：
6972.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | NS170 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | PhosphorylatedNeurofilament(pNF-H)SandwichELISAKit |
| BackgroundInformation | TheChemiconPhosphorylatedNeurofilamentH(pNF-H)kitisasandwichenzymeimmunoassay(EIA),whichmeasurespNF-H.ThekitwillmeasurepNF-Hfromavarietyofmammalianspeciesincludinghuman,rat,mouse,bovine,porcine,andfeline. Neurofilamentsarethe10nmdiameterfilamentswhichmakeupthemajorpartoftheCytoskeletonofneuronsandareparticularlyconcentratedinlargerdiameteraxons.Theybelongtotheintermediateor10nmfilamentprotein/genesuperfamilywhichalsoincludesthekeratins,themajorstructuralproteinsofskin.NeurofilamentsaregenerallyregardedasbeingcomposedofthreemajorproteinsubunitsreferredtoasNF-L,NF-MandNF-H.NF-Listhe"low"or"light"subunit,NF-Misthe"medium"or"middle"subunitandNF-Histhe"heavy"or"high"subunit.ThisnomenclatureisbasedontheapparentsizeoftheseproteinsonSDS-PAGEgels;NF-Listypicallyabout68kDa,NF-Misabout150kDaandNF-Hisabout200kDa.Thesethreeproteins,referredtoastheneurofilamenttriplet,werediscoveredinthe1970sasaresultofstudiesofaxonallytransportedproteins(HoffmanandLasek1975).Afourthprotein,α-internexin,a.k.aNF66,waslaterdiscoveredasaproteinco-purifiingwiththeneurofilamenttripletproteins(PachterandLiem1985).Afifthprotein,peripherin,isfoundinsomeneurofilamentsalongwiththeotherfourproteins,mostlyintheperipheralnervoussystem(Portieretal.1983).Neurofilamentsaremajorproteinsofneuronsandareparticularlyconcentratedinaxons.TheproteinNF-Hhassomeveryunusualproperties.Firstlyitcontains50-60backtobackhexa,heptaoroctapeptiderepeatseachcontainingthesequenceLysine-Serine-Proline(KSP).Theserineresiduesinthesepeptiderepeatsarephosporylatedinvivo,andinaxonalneurofilaments,thesesitesareheavilyphosphorylated.ThephosphorylatedformsofNF-Harealsoquiteresistanttoproteases,suggestingthatthisveryabundantproteinmightbeparticularlyresistanttoproteotyticcleavageuponreleasefromdamagedanddiseasedaxons.Thismeansthatdetectionofthisproteinincerebrospinalfluid(CSF)andbloodwouldprovideinformationaboutthedegreeofaxonalinjurywhichhasoccurred(reviewedinPetzold2005).Asrecentlyshown,thisproteincanbedetectedinquitelargeamountsfollowingexperimentalspinalcordandbraininjuryinrats(Shawetal.2005).Levelsofgreaterthan100ng/mlofpNF-Hweredetectableinbloodfollowingseriousspinalcordinjuryandlowerbutstilleasilydetectablelevelswereseeninbloodofanimalsgivenexperimentalbraininjury.Morerecentstudieshaverevealedconsiderableamountsofthisproteininthebloodofmicetransgenicformutationsofhumancopper/zincsuperoxidedismutase1whichareassociatedwithamyotrophiclateralsclerosis(ALS).ThesemicedevelopanaxonaldegenerationpathologysimilartothatseeninhumanswithALS,andbloodpNF-Hlevelscanbeusedtomonitorthis.Interestingly,pNF-Hisdetectablebeforetheonsetofobviousdiseasesymptoms.OtherstudiesshowthatpNF-Hcanbedetectedintissuehomogenates.OtherexperimentsshowthatpNF-Hcanbedetectedintheplasmaofhumanssufferingfromopticneuritis(Petzoldetal2004)andinelevatedlevelsinthecerebrospinalfluid(CSF)ofindividualssufferingfrombraintumorsandstroke(PetzoldandShaw,unpublished). TestPrincipleWiththeChemiconpNF-Hassaysystem,chickenpolyclonalantibodiesgeneratedagainstpNF-H,arepre-coatedontoa96-wellplateandareusedtocapturepNF-Hfromasample.CapturedpNF-HisdetectedusingpNF-Hspecificrabbitpolyclonalantibodiesandagoatanti-rabbitalkalinephosphataseconjugate.AfteradditionofthesubstratesolutiontheamountofpNF-Hisdetermined.ThestandardcurvedemonstratesadirectrelationshipbetweenOpticalDensity(OD)andpNF-Hconcentration:i.e.,thehighertheODthehigherthepNF-Hconcentrationinthesample. ApplicationTheChemiconpNF-HkitisdesignedtomeasuretheamountofpNF-HintissuehomogenatesandBIOLOGicalfluid(serum,plasma,andserum-free)samplesfrommouseandrat.Thereareenoughreagentsincludedinthiskitforone96-wellimmuno-assayplate.Runningduplicatewellsforsamplesandstandardsisrecommended. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Pipettors&tipscapableofaccuratelymeasuring10-1000μL 3.GraduatedSEROlogicalpipettes 4.96-wellmicroplatereadercapableofmeasuringabsorbanceat405nm 5.Graphpaperformanualplottingofdata 6.1.5mLtubes 7.Mechanicalvortex 8.Two1litercontainers 9.Plateshaker(optional) 10.3NNaOH(optional) 11.Distilledordeionizedwater. |
| ProductInformation | |
|---|---|
| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Priortousemaintainthekitcomponentsat2°-8°Cand-20°C,asindicated,untilexpirationdate. 1.WashBuffer-AfterdilutionmaintaintheWashSolutionat2°-8°Cforuptoonemonthoruntilexpirationdate. 2.Tween-20™-Storeatroomtemperatureuponreceiptofkit. 3.ElisaAssayBuffer-Aliquotasrequiredtoavoidrepeatedfreeze-thawcycles.Afterthawing,maintaintheElisaAssayBufferat2°-8°Cforuptoonemonth,oruntilexpirationdate. 4.ImmunoplatePre-coatedwithChickenanti-pNF-HPolyclonalAntibody-Unusedstripwellsmaybereturnedtothefoilpouchandstoredat2°-8°Cforuptoonemonth,oruntilexpirationdate. |
| Applications | |
|---|---|
| Application | ThePhosphorylatedNeurofilamentH(pNF-H)kitisasandwichenzymeimmunoassay(EIA),whichmeasurespNF-H. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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