Millipore/MAB5448 |抗神经丝NF-H抗体，克隆TA51/MAB5448/1 mL
市场价：
¥6780.00
美元价：
4068.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5448 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-NeurofilamentNF-HAntibody,cloneTA51 |
| AlternateNames |
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| ProductInformation | |
|---|---|
| Format | CultureSupernatant |
| Presentation | Unpurifiedtissueculturesupernatantfromaperfusionsystem,filteredthrougha0.2μmembranepriortovialing.Productcontains20%FBSandCiprofloxacinatfinalconcentrationof10μg/mL. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforupto12monthsafterdateofreceipt.Avoidrepeatedfreeze/thawcycles. |
| Applications | |
|---|---|
| Application | Anti-NeurofilamentNF-HAntibody,cloneTA51isanantibodyagainstNeurofilamentNF-HforuseinELISA,IP,WB,IH. |
| KeyApplications |
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| ApplicationNotes | Westernblot:Identifies~200kDabandinreducedsamples.UseTrisbasedbuffersforbestresultsasepitopeisphosphatedependent;DifferentregionsoftheCNSweredissectedoutimmediatelyandhomogenizedinBUSTbuffer(8Murea,0.5%SDS,2%13-mercaptoethanol,and50mMTris,pH7.4)ataratioofiml/100mgwettissue.ThetissuesUSPensionsweresonicatedwellandcentrifugedinaTL-100ultracentrifuge(BeckmanInstruments,Fullerton,CA)at100,000g for1hat25°C,andtheproteinconcentrationsinthesupernatantsweredetermined.Proteinswereseparatedon7.5%SDS-PAGEgels,{Tu.Petal(1995)JCellBiol129:1629-1640} Immunohistochemistryonfrozensections;noteffectiveonparaffintissuesinoriginalwork.{Cardenetal,1987}. Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Epitope | carboxyterminalphosphorylationdependent,withinthemultiphosphorylationrepeatinNFH |
| Clone | TA51 |
| Host | Rat |
| Specificity | NeurofilamentH(NF-H),carboxyterminal(phosphatedependent(+).CloneisspecificforphosphorylatedNF-H{Cardenetal(1987)7(11):3489}. |
| Isotype | IgG |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12036#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.NF-HhasanimportantfunctioninmatureaxonsthatisnotsubservedbythetwosmallerNFproteins. SIZE:1026aminoacids;112480Da PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFHisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFHresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction. DISEASE:SwissProt:P12036#DefectsinNEFHareacauseofsusceptibilitytoamyotrophiclateralsclerosis(ALS)[MIM:105400].ALSisaneurodegenerativedisorderaffectingupperandlowermotorneurons,andresultinginfatalparalysis.Sensoryabnormalitiesareabsent.Deathusuallyoccurswithin2to5years.Theetiologyislikelytobemultifactorial,involvingbothgeneticandenvironmentalfactors. SIMILARITY:SwissProt:P12036##Belongstotheintermediatefilamentfamily. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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