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Millipore/MAB5256 | Anti-Neurofilament 200 kDa Antibody, clone NE14/MAB5256/40 µg
Millipore/MAB5256 | Anti-Neurofilament 200 kDa Antibody, clone NE14/MAB5256/40 µg
市场价：
¥7400.00
美元价：
4440.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB5256 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-Neurofilament200kDaAntibody,cloneNE14 |
| BackgroundInformation | NeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Presentation | Purifiedimmunoglobulin.Liquid.Buffer=0.02MPhosphatebuffer,0.25MNaClcontaining0.1%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainrefrigeratedat2-8°Cinundilutedaliquotsforupto6months.DONOTFREEZE |
| Applications | |
|---|---|
| Application | DetectNeurofilament200kDausingthisAnti-Neurofilament200kDaAntibody,cloneNE14validatedforuseinIH. |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistry:5-10μg/mL(Seebelowprotocol.) Optimalworkingdilutionsmustbedeterminedbyenduser. ImmunohistochemistryProtocolforAnti-Neurofilament200kD Idealspecimensareobtainedfromfrozensectionsfromshock-frozentissuesamples.Thefrozensectionsaredriedintheairandthenfixedwithacetoneat-15to-25°Cfor10min.Excessacetoneisallowedtoevaporateat15-25°C.Materialfixedinalcoholandembeddedinparaffincanalsobeused,see(Altmannsbergeretal.,1982).Theantibodyappearstoreactwithtissuefixedinformaldehydeforashorttime(10min)(Debusetal.,1983).Otherfixationconditionsmustbefirsttestedbytheinvestigator. Itisadvantageoustoblockunspecificbindingsitesbyoverlayingthesectionswithfetalcalfserumfor20-30minat15-25°C.Excessoffetalcalfserumisremovedbydecantingbeforeapplicationoftheantibodysolution.Cytocentrifugepreparationsofsinglecellsorcellsmearsarealsofixedinacetone.Thesepreparationsshould,however,notbedriedintheair.Instead,theexcessacetoneisremovedbybrieflywashinginphosphate-bufferedsaline(PBS). Furthertreatmentisthenasfollows: Overlaythepreparationwith10-20μLantibodysolutionandincubateinahumidchamberat37°Cfor1h. DiptheslidebrieflyinPBSandthenwash3timesinPBSfor3min(usefreshPBSeachtime) Wipethemarginsofthepreparationdryandoverlaythepreparationwith10-20μLofananti-mouseIg-FITCoranti-mouseIg-PODantibodyandallowtoincubatefor1hat37°Cinahumidchamber. Washtheslideasdescribedabove. Thepreparationmustnotbeallowedtodryoutduringanyofthesteps. Ifusinganindirectimmunofluorescencetechnique,thepreparationshouldbeoverlaidwithasuitableembeddingmedium(e.g.Moviol,Hoechst)andexaminedunderthefluorescencemicroscope.IfaPOD-conjugatehasbeenusedasthesecondaryantibody,thepreparationshouldbeoverlaidwithasubstratesolution(seebelow)andincubatedat15-25°CuntilaclearlyvisIBLered-browncolordevelops.Anegativecontrol(e.g.onlythesecondaryantibody)shouldremainunchangedincolorduringthisincubationperiod.Subsequently,thesubstrateiswashedoffwithPBSandthepreparationisstained,ifdesired,withhemalumstainforabout1min.ThehemalumsolutioniswashedoffwithPBS,thepreparationisembeddedandexamined. Substratesolutions: Aminoethyl-carbazole: Dissolve2mg3-amino-9-ethylcarbazolewith1.2mLdimethylsulfoxideandadd28.8mL0.05MTris-HCl,pH7.3,and20μL3%H2O2(w/v).Preparesolutionfreshlyeachday. Diaminobenzidine: Dissolve25mg3,3"-diaminobenzidinewith50mL0.05MTris-HCl,pH7.3,andadd40μL3%H2O2(w/v).Preparesolutionfreshlyeachday. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Purifiedneurofilamentpolypeptides(Debusetal.,1983). |
| Clone | NE14 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Theantibodyreactswithneurofilament200kD. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12036#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.NF-HhasanimportantfunctioninmatureaxonsthatisnotsubservedbythetwosmallerNFproteins. SIZE:1026aminoacids;112480Da PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFHisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFHresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction. DISEASE:SwissProt:P12036#DefectsinNEFHareacauseofsusceptibilitytoamyotrophiclateralsclerosis(ALS)[MIM:105400].ALSisaneurodegenerativedisorderaffectingupperandlowermotorneurons,andresultinginfatalparalysis.Sensoryabnormalitiesareabsent.Deathusuallyoccurswithin2to5years.Theetiologyislikelytobemultifactorial,involvingbothgeneticandenvironmentalfactors. SIMILARITY:SwissProt:P12036##Belongstotheintermediatefilamentfamily. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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