微孔/MAB1592 |抗神经丝H&M抗体，磷酸化，克隆NP1/MAB1592/100µ；L
市场价：
¥6980.00
美元价：
4188.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB1592 |
| Replaces | 04-1061 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-NeurofilamentH&MAntibody,phosphorylated,cloneNP1 |
| BackgroundInformation | NeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection. |
| ProductInformation | |
|---|---|
| Format | Ascites |
| Presentation | Liquid.Containsnopreservative. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainat-20°Cinundilutedaliquotsforuptosixmonths.Avoidrepeatedfreeze/thawcycles. |
| Applications | |
|---|---|
| Application | DetectNeurofilamentH&MusingthisAnti-NeurofilamentH&MAntibody,phosphorylated,cloneNP1validatedforuseinELISA,FC,RIA,WB,IC,IH(P). |
| KeyApplications |
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| ApplicationNotes | Westernblot:recognizes,200kDa,and150kDaNF-HandNF-Mbandsprimarily.Stainingisremovedwithphosphatasetreatment.BecauseNF-Lalsocontainslysine-serinePO4sites,NP1maycrossreactwithNF-Laswell,butthishasnotbesubstantiatedintraditionalwesterns. Immunohistochemistry.Worksonparaffinembedded,formalinfixedandfrozenacteonefixedtissuesections.1:50-1:500forimmunofluorescence. ImmunocytochemistryonPC12,TERA-2andotherneuronallines. FACSELISA/RIA Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Bovineneurofilaments |
| Epitope | phosphorylated |
| Clone | NP1 |
| Host | Mouse |
| Specificity | RecognizestheKSPrepeatsofthephosphorylatedformsofneurofilament-M(NF-M)andneurofilament-H(NF-H),becauseNF-LdoescontainafewoftheseLysine-serine-PO4-prolinerepeatssomecrossreactivitytoNF-Lmaybeseenatveryhighantibodyconcentrations. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12036#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.NF-HhasanimportantfunctioninmatureaxonsthatisnotsubservedbythetwosmallerNFproteins. SIZE:1026aminoacids;112480Da PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFHisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFHresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction. DISEASE:SwissProt:P12036#DefectsinNEFHareacauseofsusceptibilitytoamyotrophiclateralsclerosis(ALS)[MIM:105400].ALSisaneurodegenerativedisorderaffectingupperandlowermotorneurons,andresultinginfatalparalysis.Sensoryabnormalitiesareabsent.Deathusuallyoccurswithin2to5years.Theetiologyislikelytobemultifactorial,involvingbothgeneticandenvironmentalfactors. SIMILARITY:SwissProt:P12036##Belongstotheintermediatefilamentfamily. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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