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Millipore/17-600 | ChIPAb+CREB-芯片验证抗体和引物集，兔单克隆/17-600/25分析25分析pe
Millipore/17-600 | ChIPAb+CREB-芯片验证抗体和引物集，兔单克隆/17-600/25分析25分析pe
市场价：
¥8000.00
美元价：
4800.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-600 |
| BrandFamily | Upstate |
| TradeName |
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| Description | ChIPAb+CREB-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal |
| Overview | EverylotoftheChIPAb+lineofantibodiesisindividuallyvalidatedforchromatinprecipitation,inordertoguaranteesuccessfulChIPassayseverytime.Eachantibodyincludesacontrolprimersetforperformanceconfirmation.CREBantibodyandthenegativecontrolantibody(rabbitnormalIgG)canbeusedtodemonstratethattheCREBantibodyisfunctionallyvalidatedintheprecipitationofCREBassociatedchromatin. TheqPCRprimersincludedflanktheCREBbindingsiteinhumanNR4A2promoter. |
| AlternateNames |
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| BackgroundInformation | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+CREB,antibody/primersetincludestheCREB,antibody,anegativecontrolantibody(purifiedrabbitIgG),andqPCRprimerswhichamplifya64bpregionflankingtheCREofthehumancFosgene.TheCREBandnegativecontrolculturedsupernatantaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofCREBassociatedchromatin. |
| ProductInformation | |
|---|---|
| Components |
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| Format | CultureSupernatant |
| Control |
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| Presentation | 25assaysperset.Recommendeduse:~10μLantibodyperchromatinimmunoprecipitation(dependentuponbiologicalcontext). |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| BiologicalInformation | |
|---|---|
| Immunogen | CREBpurifiedantibodyismadeagainstaKLH-conjugatedsyntheticpeptidecorre-spondingtoaminoacids5-24ofhumanCREB. |
| Epitope | a.a.5-24 |
| Clone | NL904 |
| Host | Rabbit |
| Specificity | RecognizesCREB,MW~43kDa. |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Human,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity. SUBUNITSTRUCTURE:InteractswithPPRC1.BindsDNAasadimer.ThisdimerisstABIlizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity. SUBCELLULARLOCATION:Nucleus. PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR. INVOLVEMENTINDISEASE:AchromosomalaberrationinvolvingCREB1isassociatedwithangiomatoidfibroushistiocytoma(AFH)[MIM:612160].Translocationt(2;22)(q33;q12)withCREB1generatesaEWSR1/CREB1fusiongenethatismostcommongeneticabnormalityinthistumortype. SEQUENCESIMILARITIES:BelongstothebZIPfamily.Contains1bZIPdomain.Contains1KID(kinase-inducIBLe)domain. |
| MolecularWeight | ~43kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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