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密理博/17-10131 | ChIPAb+™ 磷酸化CREB（Ser133）-芯片验证抗体和引物集/17-10131/25分析25分析
密理博/17-10131 | ChIPAb+™ 磷酸化CREB（Ser133）-芯片验证抗体和引物集/17-10131/25分析25分析
市场价：
¥8000.00
美元价：
4800.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-10131 |
| TradeName |
|
| Description | ChIPAb+™Phospho-CREB(Ser133)-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+Phospho-CREB(Ser133)setincludesthePhospho-CREB(Ser133)antibody,anegativecontrolnormalrabbitIgG,andqPCRprimerswhichamplifya64bpregionofhumancFosCRE.ThePhospho-CREB(Ser133)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofPhospho-CREB(Ser133)-associatedchromatin. |
| AlternateNames |
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| BackgroundInformation | CREBisaβ-ZIPtranscriptionfactorthatactivatestargetgenesthroughcAMPresponseelements.CREBisabletomediatesignalsfromnumerousphysiologicalstimuli,resultinginregulationofabroadarrayofcellularresponses.WhileCREBisexpressedinnumeroustissues,itplaysalargeregulatoryroleinthenervoussystem.CREBisbelievedtoplayakeyroleinpromotingneuronalsurvival,precursorproliferation,neuriteoutgrowthandneuronaldifferentiationincertainneuronalpopulations.CREBpromotesoutgrowthanddifferentiationasamediatorofneurotrophinpathways.CREBisabletoselectivelyactivatenumerousdownstreamgenesthroughinteractionswithdifferentdimerizationpartners.CREBisactivatedbyphosphorylationatSer133byvarioussignalingpathwaysincludingErk,Ca2+andstresssignaling. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Anti-phospho-CREB(Ser133)(rabbitpolyclonal),PartNo.CS204400.Onevialcontaining100µgofaffinitypurifiedrabbitserumin0.1MTris-Glycine(pH7.4)0.15MNaCl,and0.05%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C. NormalRabbitIgG,PartNo.PP64B.Onevialcontaining125µgRabbitIgGin125µLstoragebuffercontaining0.05%sodiumazide. Storeat-20°C. ChIPPrimerscFosCRE,PartNo.CS203203.Onevialcontaining75μLof5μMofeachprimerspecificforthecFosCRE.Storeat-20°C. FOR:GGCCCACGAGACCTCTGAGACA REV:GCCTTGGCGCGTGTCCTAATCT |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BiologicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedsyntheticpeptidecorrespondingtotheaminoacidsincludingandencompassingphosphorylatedSer133ofCREB. |
| Epitope | phospho-Ser133 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | Recognizesp43phosphorylatedCREB.Mayalsorecognizep30andp38kDaproteins.Thep30&p38proteinsmaybephosphorylatedCREMandATF-1respectively,sincethetwoproteinssharesignificanthomologytothephosphorylatedCREBimmunizingpeptide. |
| Isotype | IgG |
| SpeciesReactivity |
|
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms. |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | PurifiedbyproteinA-SepharosefollowedbyimmunoadsorbantchromatographyusinganunphosphorylatedCREBaffinitygelcolumn. |
| UniProtNumber | |
| UniProtSummary | FUNCTION:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity. SUBUNIT:InteractswithPPRC1.BindsDNAasadimer.Thisdimerisstabilizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity. SUBCELLULAR:Nucleus. PMT:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:BelongstothebZIPfamily. Contains1bZIPdomain. Contains1KID(kinase-inducIBLe)domain |
| MolecularWeight | DetectsCREBonlywhenphosphorylatedonSer133(~43kDa).MayrecognizeATF-1(~38kDa)andCREM(~30kDa)asthetwoproteinssharesignificanthomologytothephosphorylatedimmunizingpeptide. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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