Millipore/06-863 | Anti-CREB Antibody/06-863/200 µg
市场价：
¥6360.00
美元价：
3816.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 06-863 |
| Replaces | 04-218 |
| BrandFamily | Upstate |
| TradeName |
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| Description | Anti-CREBAntibody |
| AlternateNames |
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| BackgroundInformation | CyclicAMP-responsiveelement-bindingprotein1(UniProtP16220;alsoknownascAMP-responsiveelement-bindingprotein1,CREB-1)isencodedbytheCREB1gene(GeneID1385)inhuman.CREBisatranscriptionfactorthatbindsthecAMPresponseelements(CRE)andregulatesthetranscriptionofthedownstreamgenes.CREBactivityisregulationviaphosphorylationbyvariouskinases,includingPKA,andCa2+/calmodulin-dependentproteinkinases.GenesknowntoberegulatedbyCREBincludec-fos,BDNF,tyrosinehydroxylase,neuropeptides(suchassomatostatin,enkephalin,VGF,andcorticotropin-releasinghormone),aswellasthecircADIanclockgenesPER1andPER2.CREBiscriticalformemoryformationacrossawiderangeofspecies. |
| ProductInformation | |
|---|---|
| Format | ProteinAPurified |
| Control |
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| Presentation | Purifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidewith30%glycerol. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| Applications | |
|---|---|
| Application | Anti-CREBAntibody,Cat.No.06-863,isahighlyspecificrabbitPolyclonalantibody,thattargetsCREBandhasbeentestedinChromatinImmunoprecipitation(ChIP),ChIP-seq,ElectrophoreticMobilityShiftAssay(EMSA),Immunoprecipitation,andWesternBlotting. |
| KeyApplications |
|
| ApplicationNotes | ImmunoprecipitationAnalysis:10µgfromarepresentativelotimmunoprecipitatedCREBin500µgofHeLanuclearextract. ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedanenhancedCREBoccupancyattheLYPD3promoterinLKB1-deficientTE-4humanesophagealcancercells(Gu,Y.,etal.(2012).Oncogene.31(4):469–479). ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedCREBassociationattheRNASET2sitestargetedbyT-cellLeukemiaVirustype1(HTLV-1)encodedTaxproteinusingHTLV-1-infectedhumanT-celllines,MT-2,C8166/45andSLB-1(Polakowski,N.,etal.(2011).Viruses.3(8):1485-500). ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedCREBoccupancyatthehBDNFpromoterIVusingpostmortemhumanparietalcortextissuechromatinpreparations(Pruunsild,P.,etal.(2011).J.Neurosci.31(9):3295-3308). ChromatinImmunoprecipitation(ChIP)Analysis:ArepresentativelotdetectedCREBoccupancyatthemiR-9-2promoterinSK-N-BEhumanneuroblastomacells(Laneve,P.,etal.(2010).Nucl.AcidsRes.38(20):6895-6905). ChIP-seqAnalysis:ArepresentativelotidentifiedCREBtargetgenesinmurinespermatogoniaderivedGC1-spgcellsbyChIP-seqanalysis(Martianov,I.,etal.(2010).BMCGenomics.11:530). WesternBlottingAnalysis:RepresentativelotsdetectedCREBinlysatesfromculturedembryonicratstriatalneuronsandPC12pheochromocytomacells(Hutchinson,A.N.,etal.(2012).Neuropsychopharmacology.37(2):321-337;Maharjan,S.,etal.(2010).J.NeuRochem.112(1):42-55). WesternBlottingAnalysis:RepresentativelotsdetectedCREBinmouseproximalcaputepididymis-1(PC-1)celllysateandhippocampustissuehomogenates(Hamzeh,M.,andRobaire,B.(2011).J.Endocrinol.209(1):55-64;Zhou,Z.,etal.(2009).Neuropharmacology.56(1):81-89). WesternBlottingAnalysis:ArepresentativelotdetectedCREBinSK-N-BEhumanneuroblastomacelllysate(Laneve,P.,etal.(2010).Nucl.AcidsRes.38(20):6895-6905). ElectrophoreticMobilityShiftAssay(EMSA):ArepresentativelotcausedasupershiftofDNA-CREBcomplexinEMSAusingradiolabeledoligonucleotidecontaininghBDNFpromoterICREB-bindingsequenceandlysatesfromKCl-treatedprimaryratneurons(Pruunsild,P.,etal.(2011).J.Neurosci.31(9):3295-3308). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugated,syntheticpeptide(CSGAENQQSGDAAVTEAENQQ)correspondingtoa.a.5-24ofhumanCREB.Theimmunizingsequenceisidenticalinpig,shares19of20residueswithmouseandbovineand18of20residueswithrat. |
| Epitope | Aminoacids5-24. |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | ThisantibodyrecognizesCREB. |
| Isotype | IgG |
| SpeciesReactivity |
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| SpeciesReactivityNote | Human,Mouse,Rat.PredictedtoreactwithCanine,Horse,Porcinebasedon100%sequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms. |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P16220#ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE. SIZE:341aminoacids;36688Da SUBUNIT:BindsDNAasadimer.InteractsPPRC1andwithHTLV-1Tax-1. SUBCELLULARLOCATION:Nucleus. PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPandEP300binding(Bysimilarity).PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P16220##BelongstothebZIPfamily.&Contains1bZIPdomain.&Contains1KID(kinase-inducIBLe)domain. |
| MolecularWeight | ~43kDaobserved. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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