Millipore/01-185 | VEGF蛋白，重组人/01-185/10µ；G
市场价：
¥6940.00
美元价：
4164.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 01-185 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | VEGFProtein,Humanrecombinant |
| ProductInformation | |
|---|---|
| Formulation | |
| Presentation | 10µgofVEGFlyophilizedproteinfrombuffercontaining25mMHEPESand150mMNaCl,pH7.0.Carrierfree. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Thelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewateror25mMHEPESand150mMNaCl,pH7.0.Reconstitutedmaterialcanbestoredat2°-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles. Thisproductisacidstable.Diluteineither0.1%TFAor4mMHCl,containing25ugBSAperugVEGFtomaintainstABIlity.Reducingreagents(suchasdithiothrietolor2-mercaptoethanol)inhibitVEGFactivity. |
| Applications | |
|---|---|
| Application | ThehumanrecombinantVEGFProteinisavailableina10µgformat. |
| KeyApplications |
|
| ApplicationNotes | VEGFProliferationAssay 1.Priortouseinthisassay,maintainHUVEcellsinF-12Kmediumsupplementedwith10%(v/v)FBS,1%penicillin-streptomycin-glutamine,0.1mg/mlheparinand0.05mg/mlendothelialcellgrowthsupplement(ECGS). 2.ForassayofVEGF,subcultureHUVEcellsintoa96-wellplateatadensityof4,000cellsperwellinthegrowthmediumovernight. 3.AddVEGFtoafinalconcentrationrangeof0.2,0.4,0.8,1.6,3.2,6.2,12.5,25and50ng/ml.AddVEGFdiluenttoonesetofwellsasthenegativecontrol. 4.Fourdayslater,add20ulofPromegaSubstrateCellTiter96AqueousOneSolutionReagenttoeachwell. 5.Incubateat37Cfor2hrsandreadatOD490nm. |
| BIOLOGicalInformation | |
|---|---|
| Source | RecombinanthumanDNAexpressedinbaculovirusinfectedSf9cells |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms. |
| GeneSymbol |
|
| PurificationMethod | Sterilizedthrougha0.2micronmembranefilter |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145. SIZE:232aminoacids;27042Da SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity). SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin. TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon. SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily. |
| StemCellType |
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| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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