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当前位置: 首页 > 产品中心 > Functional_antibody > 密理博/AB938 |抗5-羟色胺抗体/AB938/500µ;L
商品详细密理博/AB938 |抗5-羟色胺抗体/AB938/500µ;L
密理博/AB938 |抗5-羟色胺抗体/AB938/500µ;L
密理博/AB938 |抗5-羟色胺抗体/AB938/500µ;L
商品编号: AB938
品牌: 密理博
市场价: ¥6580.00
美元价: 3948.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAB938
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-SEROtoninAntibody
AlternateNames
  • 5-HT
  • 5-Hydroxytryptamine
ProductInformation
FormatSerum
PresentationLiquidantiserum.Contains0.1%sodiumazide.
StorageandShippingInformation
StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze/thawcycles.
Applications
ApplicationDetectSerotoninusingthisAnti-SerotoninAntibodyvalidatedforuseinIF,IH(P).
KeyApplications
  • Immunofluorescence
  • Immunohistochemistry(Paraffin)
ApplicationNotesTISSUEEVALUATION:


Formalin-fixedandparaffinembeddedsections(5-6micronsthick)ofhumansmallintestinewereusedintheevaluationofthisantiserum.





IMMUNOPEROXIDASE


STAINING:


1.Thesectionstobestainedarehydratedtophosphatebufferedsaline(PBS,pH7.4).


2.Theantiserumdiluted1:1,000-1:2,000(inPBS)isallowedtoreactwiththetissueat37*Cfor30minutes.


3.UsingPBS,thetissueiswashedfor15minutesatroomtemperatureandtreatedsequentiallywithgoatanti-rabbitgammaglobulin(diluted1:20inPBS)andperoxidaseanti-peroxidasecomplex(diluted1:100inPBS)for30minutesat37*C.Aftereachofthesesteps,thetissueiswashedwithPBSasbefore.


4.Thefinalstageofthereactionistreatmentofthesectionwithamixtureof3,3"-diamin-benzidineHCl(0.02%)inhydrogenperoxide(0.004%).Forpreservation,thesectionsaredehydrated,clearedandmounted.





IMMUNOFLUORESCENCE


STAINING:





1.SameasStep1above.


2.Thetissueisreactedwiththeantiserum,diluted1:20-1:40in(PBS)for30minutesat37*C.


3.AfterwashingofftheexcessantiserumwithPBS,thesectionistreatedwithfluoroscein-labeledgoatanti-rabbitgammaglobulinserum(diluted1:10inPBS)for30minutesat37*C.ThetissueiswashedwithPBSandaftermountingundersaline-glycerol,isviewedwithafluorescencemicroscope.
BIOLOGicalInformation
ImmunogenTheantigenusedintheproductionofthisantiserumisserotonincovalentlyboundtobovinethyroglobulinwithcarbodiimide.
HostRabbit
SpecificityTheantiserumprovidedhasbeenevaluatedforitsABIlitytostainserotonin-containingstructuresintheileumofman.Stainingispositiveusingboththeimmunoperoxidaseorimmunofluorescenceprocedures.
SpeciesReactivity
  • Human
AntibodyTypePolyclonalAntibody
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。