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密理博/FCCS025106 | Flowcell等™ MAPK激活双检测试剂盒/FCCS025106/25测试和#47；配套元件
密理博/FCCS025106 | Flowcell等™ MAPK激活双检测试剂盒/FCCS025106/25测试和#47；配套元件
商品编号：
FCCS025106
品牌：
密理博
市场价：
¥6940.00
美元价：
4164.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | FCCS025106 |
| TradeName |
|
| Description | FlowCellect™MAPKActivationDualDetectionKit |
| Overview | Millipore’sFlowCellect™MAPKActivationDualDetectionkitincludestwodirectlyconjugatedantibodies,aphospho-specificAnti-phospho-ERK1/2(Thr202/Tyr204,Thr185/Tyr187)-PEandanAnti-ERK1/2-AlexaFluor®647conjugatedantibodytomeasuretotallevelsofERK.ThistwocolorflowcytometrykitisdesignedtomeasuretheextentofMAPKphosphorylationrelativetothetotalMAPKexpressioninanygivencellpopulation.Bydoingsuch,thelevelsofboththetotalandphosphorylatedproteincanbemeasuredsimultaneouslyinthesamecell,resultinginanormalizedandaccuratemeasurementofMAPKactivationafterstimulation.Moreover,simultaneousmeasurementofbothtotalandphospho-ERK1/2confirmstargetspecificityofthephosphorylationevent.Together,atotalandphosphoantibodyduoperformedinmultiplexprovidesanenhancedandmorereliabledetectionofthephospho:totalratiowithinamixedpopulation. AllFlowCellectkitsaresuppliedwithoptimizedfixation,permeABIlization,wash,andassaybufferstoprovideresearcherstheabilitytodetecttheactivationofERKinflowapplications.Sufficientreagentsareprovidedfor25two-colorsamples.Thekitincludesalloptimizedfluorescentlylabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis. |
| AlternateNames |
|
| BackgroundInformation | Erk(Extracellularsignal-RelatedKinase)isafamilyoftwo,highlyhomologousproteinsdenotedasErk1(p44,MAPK3)andErk2(p42,MAPK1)thatbothfunctioninthesamepathway.ThetwoproteinsareoftenreferredtocollectivelyasErk1/2orp44/p42MAPkinase.TheErkpathwayisconsideredtheclassical,canonicalMAPK(Mitogen-ActivatedProteinKinase)signalingpathway.Itisanevolutionarilyconservedpathwaythatcontrolsandisacriticalregulatorofthegrowthandsurvivalthroughthepromotionofcellproliferationandthepreventionofapoptosis.Erkisinvolvedinthecontrolofmanyfundamentalcellularprocessesincludingcellproliferation,survival,differentiation,apoptosis,motilityandmetabolism.Erkisactivatedbygrowthfactorstimulationofreceptortyrosinekinases(RTKs),GPCR,and/orintegrinstimulation.ThisactivatestheRas-Raf-MEK-Erkpathwaythatresultsinthephosphorylation/activationofErk1/2(p44/p42)ontheTxYmotif(Thr202/Tyr204andThr185/Tyr187forErk1&Erk2,respectively). Theregulationofalargenumberofcellularprocesses(suchascellproliferation/differentiation,cellsurvivalandapoptosis)isdependentontheactivationofMAPKsignalingpathway,whichcanbemeasuredbythephosphorylationofERK1/2. |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Fluorescent |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 4-8°Cforantibodiesandbuffers |
| BIOLOGicalInformation | |
|---|---|
| Host | Mouse |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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