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微孔/17-488 |磷酸化p38α（Thr180/Tyr182）STAR ELISA试剂盒/17-488/96分析
微孔/17-488 |磷酸化p38α（Thr180/Tyr182）STAR ELISA试剂盒/17-488/96分析
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-488 |
| BrandFamily | Upstate |
| TradeName |
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| Description | phospho-p38α(Thr180/Tyr182)STARELISAKit |
| BackgroundInformation | I.TESTPRINCIPLE TheUPSTATE®colorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.Thep38MAPKplateiscoatedwithaspecificmousemonoclonalp38MAPKcaptureantibodyonthemicrowellsofthe96-wellclearplate.Samplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingp38MAPKantigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-p38(Thr180/Tyr182)antibodytodetectthecapturedp38(Thr180/Tyr182)ontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader. Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardlineofdetection. II.p38BACKGROUND Thep38MAPKfamilyofSer/Thrkinaseproteinsismadeupof4isoforms,p38alpha(MAPK14andSAPK2a),p38beta(MAPK11andSAPK2b),p38delta(MAPK13,SAPK4),andp38gamma(MAPK12,SAPK3).Theotherp38isoformsshare74%,57%,and60%sequenceidentitywiththealphaisoform,respectively.AllfourisoformspossestheTGYmotifandareactivatedbythedualphosphorylationofthethreonineandtyrosineresiduesinthismotif,whichcorrespondtoThr180/Tyr182inhumanp38alpha.p38,especiallythealphaandbetaisoforms,areabletophosphorylateawidearrayoftargetsincludingnumerouskinasesincludingMAPKAP-K2,MAPKAP-K3,PRAK,MSK,andMNKaswellastranscriptionfactorsincludingSTAT1,p53,NFATp,ATF-2,Elk-1(althoughweakly),andMEF2a/c.p38MAPKfamilymembershavetrADItionallybeenassociatedwithstress,immuneresponse,andregulationofcellproliferationandapoptosis.Theyalsohavearoleinregulatingtheproductionofpro-inflammatorycytokinesandhavebeenassociatedwithdiseasessuchasasthmaandseveralautoimmunediseases,includingrheumatoidarthritis,diabetes,andinflammatoryboweldisease.JustasERKisphosphorylatedandactivatedbyMEK,p38alphaMAPKinaseisactivatedpredominantlybyitsupstreamregulatorykinasesMKK6andMKK3aswellasMKK4whichalsoactivatesJNK,butisnotasubstrateforMKK7likeJNKis. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000µL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater |
| ProductInformation | |
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| Components |
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| Detectionmethod | Colorimetric |
| StorageandShippingInformation | |
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| StorageConditions | Maintaintheunopenedkitat2-8°Cuntilexpirationdate. Precautions •Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata. •CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial. •SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed. •TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin. •TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly. |
| Applications | |
|---|---|
| Application | Thisphospho-p38α(Thr180/Tyr182)STARELISAKitisusedtomeasure&quantifyp38/SAPKlevels. |
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| BIOLOGicalInformation | |
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| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinasesactasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Thiskinaseisactivatedbyvariousenvironmentalstressesandproinflammatorycytokines.TheactivationrequiresitsphosphorylationbyMAPkinasekinases(MKKs),oritsautophosphorylationtriggeredbytheinteractionofMAP3K7IP1/TAB1proteinwiththiskinase.ThesubstratesofthiskinaseincludetranscriptionregulatorATF2,MEF2C,andMAX,cellcycleregulatorCDC25B,andtumorsuppressorp53,whichsuggesttherolesofthiskinaseinstressrelatedtranscriptionandcellcycleregulation,aswellasingenotoxicstressresponse.Fouralternativelysplicedtranscriptvariantsofthisgeneencodingdistinctisoformshavebeenreported. |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q16539#Respondstoactivationbyenvironmentalstress,pro-inflammatorycytokinesandlipopolysaccharide(LPS)byphosphorylatinganumberoftranscriptionfactors,suchasELK1andATF2andseveraldownstreamkinases,suchasMAPKAPK2andMAPKAPK5.Playsacriticalroleintheproductionofsomecytokines,forexampleIL-6.MayplayaroleinstabilizationofEPOmRNAduringhypoxicstress.IsoformMxi2activationisstimulatedbymitogensandoxidativestressandonlypoorlyphosphorylatesELK1andATF2.IsoformExipmayplayaroleintheearlyonsetofapoptosis. COFACTOR:Magnesium. SIZE:360aminoacids;41293Da SUBUNIT:Bindstoakinaseinteractionmotifwithintheproteintyrosinephosphatase,PTPRR.ThisinteractionretainsMAPK14inthecytoplasmandpreventsnuclearaccumulation.InteractswithSPAG9(Bysimilarity).InteractswithNP60. SUBCELLULARLOCATION:Cytoplasm(Bysimilarity).Nucleus(Bysimilarity). TISSUESPECIFICITY:Brain,heart,placenta,pancreasandskeletalmuscle.Expressedtoalesserextentinlung,liverandkidney.DOMAIN:SwissProt:Q16539TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-180andTyr-182,whichactivatestheenzyme.&PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain. |
| PhysicochemicalInformation | |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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