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Millipore/17-466 | phospho-JNK 1/2 (Thr183/Tyr185) STAR ELISA Kit/17-466/96 assays
Millipore/17-466 | phospho-JNK 1/2 (Thr183/Tyr185) STAR ELISA Kit/17-466/96 assays
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¥0.00
美元价：
0.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 17-466 |
| BrandFamily | Upstate |
| TradeName |
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| Description | phospho-JNK1/2(Thr183/Tyr185)STARELISAKit |
| BackgroundInformation | TestPrinciple TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsindenaturedcellextracts.TheJNK1/2plateiscoatedwithaspecificmousemonoclonalJNK1/2captureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingJNK1/2antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-JNK(Thr183/Tyr185)antibodytodetectthecapturedJNKontheplatewellthathasbeenphosphorylatedonThr183andTyr185.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardlineofdetection. JNKBackground JNK(c-JunN-terminalKinase,MAPK8)isamemberoftheMAPkinasefamily.JNKwasoriginallyfoundtobestressactivated,andassucharealsoreferredtoasthestress-relatedMAPK.FouralternativelysplicedtranscriptvariantsencodingdistinctisoformsofJNKhavebeenreported.JNKisregulatedbystressinducingsignalssuchasosmoticstressandUVrADIationaswellasproinflammatorycytokinessuchasIL-1andTNFα.JustasErk(p44/p42,MAPK)isactivatedbyitsupstreamkinaseMEK,JNKisphosphorylatedandactivatedbyMKK4andMKK7onthequintessentialMAPKactivationsequenceofThr-X-Tyr,whereXisaProfortheJNKkinasesonThr183/Tyr185forJNK1andThr221/Tyr223forJNK2.Oncephosphorylatedandactivated,JNKgoesontophosphorylatedownstreamtargetsinboththecytoplasmandthenucleus.JNKservesasatranscriptionalregulatorandisknowntophosphorylateanumberofdownstreamtargets,includingthetranscriptionfactorsc-Jun,ATF-2,andELK-1.C-Jun,amemberoftheAP-1familyoftranscriptionfactors,regulatescytokinegeneexpression.Alongtheselines,JNKinhibitorsmaybeeffectiveinthecontrolofrheumatoidarthritis(RH),anautoimmunediseaseinvolvingincreasedproductionofinflammatorycytokines.InhibitionofJNKsignalingalsoenhanceschemotherapy-inducedinhibitionoftumorcellgrowth,suggestingtheJNKsmayprovideamoleculartargetforthetreatmentofcancer.BothJNKandp38MAPKareoftensimultaneouslyactivatedinresponsetoenvironmentalchanges.Thesetwostresskinasesignalingpathwayshaveevolvedtorelayincreasinglycomplexrangesofenvironmentalstimuli.TheJNKpathwayiscriticallyinvolvedindiabetesandlevelsareabnormallyelevatedinobesity.Thecell-permeableJNKinhibitorypeptidemayhavepromiseasatherapeuticagentfordiabetes. |
| MaterialsRequiredbutNotDelivered | 1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring 1-1000μL 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanual plottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater |
| ProductInformation | |
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| Components |
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| Detectionmethod | Chromogenic |
| StorageandShippingInformation | |
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| StorageConditions | 1yearat4°Cfromdateofshipment |
| BIOLOGicalInformation | |
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| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinasesactasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Thiskinasetargetsspecifictranscriptionfactors,andthusmediatesimmediate-earlygeneexpressioninresponsetovariouscellstimuli.ItismostcloselyrelatedtoMAPK8,bothofwhichareinvolvedinUVradiationinducedapoptosis,thoughttoberelatedtothecytochromec-mediatedcelldeathpathway.ThisgeneandMAPK8arealsoknownasc-JunN-terminalkinases.Thiskinaseblockstheubiquitinationoftumorsuppressorp53,andthusitincreasesthestabilityofp53innonstressedcells.Studiesofthisgene"smousecounterpartsuggestakeyroleinT-celldifferentiation.Fouralternativelysplicedtranscriptvariantsencodingdistinctisoformshavebeenreported. |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P45984#JNK2isoformsdisplaydifferentbindingpatterns:alpha-1andalpha-2preferentiallybindtoc-Jun,whereasbeta-1andbeta-2bindtoATF2.However,thereisnocorrelationbetweenbindingandphosphorylation,whichisachievedataboutthesameefficiencybyallisoforms.JUNBisnotasubstrateforJNK2alpha-2,andJUNDbindsonlyweaklytoit. COFACTOR:Magnesium. SIZE:424aminoacids;48139Da SUBUNIT:Bindstoatleastfourscaffoldingproteins,MAPK8IP1/JIP-1,MAPK8IP2/JIP-2,MAPK8IP3/JIP-3/JSAP1andSPAG9/MAPK8IP4/JIP-4.TheseproteinsalsobindothercomponentsoftheJNKsignalingpathway. DOMAIN:SwissProt:P45984TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-183andTyr-185,whichactivatestheenzyme.Autophosphorylatedinvitro. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain. |
| PhysicochemicalInformation | |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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