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Millipore/15-107 | MAPK Pathway 1 Explorer Antibody MiniPack/15-107/3 vials/Pk
Millipore/15-107 | MAPK Pathway 1 Explorer Antibody MiniPack/15-107/3 vials/Pk
市场价：
¥4720.00
美元价：
2832.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 15-107 |
| BrandFamily | Upstate |
| TradeName |
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| Description | MAPKPathway1ExplorerAntibodyMiniPack |
| AlternateNames |
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| BackgroundInformation | PathwayExplorerAntibodyMiniPack: EachPathwayExplorerAntibodyMinipackcontainsthreerelatedantibodiesaspartofasignalingcascadeoracombinationoftotalandphosphorylatedformsofkeysignalingtargets.Eachofthethreeantibodiesare30%theoriginalpacksize.FullsizeversionsofeachofthePathwayExplorerantibodiesareavailableforsaleindividuallyunderthesamecatalognumberwiththeremovalof“SP”offofeachone(e.g.05-591SPcanbeorderedas05-591). Erk1/2: Erk(Extracellularsignal-RelatedKinase)isafamilyoftwo,highlyhomologousproteinsdenotedasErk1(p44,MAPK3)andErk2(p42,MAPK1)thatbothfunctioninthesamepathway.ThetwoproteinsareoftenreferredtocollectivelyasErk1/2orp44/p42MAPkinase.TheErkpathwayisconsideredtheclassical,canonicalMAPK(Mitogen-ActivatedProteinKinase)signalingpathway.Itisanevolutionarilyconservedpathwaythatcontrolsandisacriticalregulatorofthegrowthandsurvivalthroughthepromotionofcellproliferationandthepreventionofapoptosis.Erkisinvolvedinthecontrolofmanyfundamentalcellularprocessesincludingcellproliferation,survival,differentiation,apoptosis,motilityandmetabolism.Erkisactivatedbygrowthfactorstimulationofreceptortyrosinekinases(RTKs),GPCR,and/orintegrinstimulation.ThisactivatestheRas-Raf-MEK-Erkpathwaythatresultsinthephosphorylation/activationofErk1/2(p44/p42)ontheTxYmotif(Thr202/Tyr204andThr185/Tyr187forErk1&Erk2,respectively). Raf: TheRafproteins(A-Raf,B-Raf,Raf-1/c-Raf)areSer/ThrkinaseswithhomologytothePKCfamily,containinganN-terminalregulatorydomainandaC-terminalcatalyticdomain.Raf(MAPKinaseKinaseKinase)isthetopkinaseinthecanonicalMAPKpathway.MembersoftheRaffamilybindtoactivatedRas.Ras,initsGTP-boundactivestate,activatesthekinaseactivityofRaf.ThisresultsinRaftranslocationtotheplasmamembraneandactivation.Onceactivated,RafthenbindstoandactivatesMEK(MAPKinaseKinase)byphosphorylatingitonthetworesiduemotif.ItisthoughtthatB-RafmightbethepredominantactivatorofMEKandthatRaf-1hasaroleinprotectionagainstapoptosis;aprocessthatdoesnotrequireeitheritskinaseactivityoritsactivationofMEK.TheregulationofalargenumberofcellularprocessesaredependentupontheactivationstateofERK,sothecontrollingofthispathwaycouldhaveprofoundeffectsonvariousdiseases.ActivationofRaf-1involvesphosphorylationofSer338/339andTyr340/341.ActivatingmutationsofB-Rafthatdisruptitsauto-inhibitionloophavebeenimplicatedinanumberofcancers,includingmelanomaandcoloncancer. MEK1/MEK2: MAPKinase/ErkKinase(MEK),alternativelyknownasMKK,isatruedual-specificitykinase,inthatitphosphorylatestheMAPkinases(Erk1/2;p44/p42))onboththeThrandTyroftheactivationmotifTEY.Invitro,thetyrosinephosphorylationisfavored,whereasinvivobothphosphorylationeventsappeartooccursimultaneously.Thissuggeststhatanadditionalfactorispresentincellstofacilitatethereaction.MEK1andMEK2areactivatedbyphosphorylationoftwoserineresidues(Ser218/222inMEK1andSer222/226inMEK2),whicharesubstratesfortheRaffamilyofkinases.MutationofthephosphorylationsitesfromSertoAspcreatesaproteinwithconstitutivekinaseactivity,whichwhenexpressedincellsisabletocausetransformation. *Seefullsizeversionsforcorrespondingreferences. |
| ProductInformation | |
|---|---|
| Components |
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| Format | Purified |
| Control |
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| Presentation | 3individualtubescontainingeitherAnti-phospho-Raf-1(Ser338);orAnti-phospho-MEK1(Ser218/222)/MEK2(Ser222/226);Anti-phospho-Erk1/2 (Thr202/Tyr204,Thr185/Tyr187),recombinantcloneAW39R |
| Properties | Eachvialis30%thesizeoftheparentcatalognumber |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. |
| Applications | |
|---|---|
| Application | ThisAntibodypackcontainsAnti-phospho-Raf-1Antibody(Ser338),Anti-phospho-MEK1Antibody(Ser218/222)/MEK2(Ser222/226),Anti-phospho-Erk1/2Antibody(Thr202/Tyr204,Thr185/Tyr187),validatedforuseinIF,Flow,WB,Multiplexing |
| KeyApplications |
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| ApplicationNotes | 05-797RSP:ImmunofluorescenceAnalysis:A431cellswerefixed,permABIlizedanddualstainedwithanti-phospho-Erk. FlowCytometryAnalysis:Jurkatcellswerefixed,permeablized,andstainedwithanti-phospho-Erk1/2(Thr202/Tyr204,Thr185/Tyr187). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | 05-797RSP:PhosphorylatedpeptideencompassingtheTxYmotifintheactivationloopofErk1andErk2.;05-747SP:KLH-conjugated,syntheticpeptidecontaining…DpSMANpSF…inwhichpScorrespondstophosphorylatedaminoacids218and222ofhumanMEK1.Theimmunizingsequenceishighlyconserved.;05-538SP:KLH-conjugated,syntheticpeptideencompassingandincludingphosphorylatedSer338ofhumanRaf-1.Theimmunizingsequenceisidenticalinratandchickenandhas12/13identicalaminoacidsinXenopus. |
| Epitope | Pleaseseerelevantdatasheet. |
| Clone | 05-797RSP:AW39R |
| Concentration | 05-538SP:1mg/mL |
| Specificity | 05-797RSP:RecognizesErk1&2onlywhenduallyphosphorylatedonitsTxYactivationmotif.;05-747SP:PhosphorylatedMEK1/MEK2;05-538SP:RecognizesRaf-1phosphorylatedatSerine338 |
| SpeciesReactivity |
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| SpeciesReactivityNote | 05-538SP:Mouseandhuman. Predictedtocrossreactwithrat,chickenandXenopusbasedonsequencehomology. |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | Raf-1: FUNCTION:Involvedinthetransductionofmitogenicsignalsfromthecellmembranetothenucleus.PartoftheRas-dependentsignalingpathwayfromreceptorstothenucleus.ProtectscellsfromapoptosismediatedbySTK3. CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein. COFACTOR:Binds2zincionspersubunit. SUBUNITSTRUCTURE:InteractswithRasproteins.TheinteractionwithRasisantagonizedbyRIN1.WeaklyinteractswithRIT1Bysimilarity.InteractswithSTK3,whichinhibitsitspro-apoptoticactivity. TISSUESPECIFICITY:Inskeletalmuscle,isoform1ismoreabundantthanisoform2. POST-TRANSLATIONALMODIFICATION:PhosphorylateduponDNAdamage,probablybyATMorATR.PhosphorylationatThr-269increasesitskinaseactivity. INVOLVEMENTINDISEASE:DefectsinRAF1arethecauseofNoonansyndrometype5(NS5)[MIM:611553].Noonansyndrome(NS)isadisordercharacterizedbydysmorphicfacialfeatures,shortstature,hypertelorism,cardiacanomalies,deafness,motordelay,andableedingdiathesis.Itisageneticallyheterogeneousandrelativelycommonsyndrome,withanestimatedincidenceof1in1000-2500livebirths.Ref.24Ref.25 DefectsinRAF1arethecauseofLEOPARDsyndrometype2(LEOPARDsyndrome-2)[MIM:611554].LEOPARDsyndromeisanautosomaldominantdisorderallelicwithNoonansyndrome.TheacronymLEOPARDstandsforlentigines,electrocardiographicconductionabnormalities,ocularhypertelorism,pulmonicstenosis,abnormalitiesofgenitalia,retardationofgrowth,anddeafness. MEK1: FUNCTION:CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases. CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein. ENZYMEREGULATION:Activatedbyphosphorylation. SUBUNIT:InteractswithMORG1(Bysimilarity).InteractswithYersiniayopJ. PTM:PhosphorylationonSer/ThrbyMAPkinasekinasekinases(RAForMEKK1)regulatespositivelythekinaseactivity. PTM:AcetylationbyYersiniayopJpreventsphosphorylationandactivation,thusblockingtheMAPKsignalingpathway. DISEASE:DefectsinMAP2K1areacauseofcardiofaciocutaneoussyndrome(CFCsyndrome)[MIM:115150];alsoknownascardio-facio-cutaneoussyndrome.CFCsyndromeischaracterizedbyadistinctivefacialappearance,heartdefectsandmentalretardation.Heartdefectsincludepulmonicstenosis,atrialseptaldefectsandhypertrophiccardiomyopathy.Someaffectedindividualspresentwithectodermalabnormalitiessuchassparse,friablehair,hyperkeratoticskinlesionsandageneralizedichthyosis-likecondition.TypicalfacialfeaturesaresimilartoNoonansyndrome.Theyincludehighforeheadwithbitemporalconstriction,hypoplasticsupraorbitalridges,downslantingpalpebralfissures,adepressednasalbridge,andposteriorlyangulatedearswithprominenthelices.TheinheritanceofCFCsyndromeisautosomaldominant. SIMILARITY:Belongstotheproteinkinasesuperfamily.STESer/Thrproteinkinasefamily.MAPkinasekinasesubfamily. SIMILARITY:Contains1proteinkinasedomain. Erk2: FUNCTION:CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases. CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein. ENZYMEREGULATION:ActivatedbyphosphorylationontyrosineandthreonineinresponsetoinsulinandNGF.Bothphosphorylationsarerequiredforactivity SUBUNITSTRUCTURE:InteractswithMORG1Bysimilarity.BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithitssubstratesHSF4andARHGEF2.InteractswithNISCH. DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme. |
| MolecularWeight | 05-797RSP:42and44kDa;05-747SP:45kDa;05-538SP:74kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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