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Millipore/14-173M | MAP Kinase 2/Erk2 Protein, active, mouse, 250 µg/14-173M/250 µgAlso available in 10 μg size (c
Millipore/14-173M | MAP Kinase 2/Erk2 Protein, active, mouse, 250 µg/14-173M/250 µgAlso available in 10 μg size (c
市场价：
¥50860.00
美元价：
30516.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 14-173M |
| BrandFamily | Upstate |
| TradeName |
|
| Description | MAPKinase2/Erk2Protein,active,mouse,250µg |
| Overview | N-terminalGST-tagged,recombinantMAPKinase2,expressedinE.colicells;activatedusingMEK1(Catalog#14-429). |
| ProductInformation | |
|---|---|
| Presentation | 50mMTris-HCl,pH7.5,150mMNaCl,0.1mMEGTA,0.03%Brij-35,50%glycerol,1mMbenzamidine,0.2mMPMSF,0.1%2-mercaptoethanol. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storeinundilutedaliquotsat-20°Cforuptooneyearfromthedateofreceipt.Avoidrepeatedefreeze-thawcycles. |
| Applications | |
|---|---|
| Application | Active,N-terminalGST-tagged,recombinantMAPKinase2,expressedinE.colicells,activatedusingMEK1.ForuseinKinaseAssays. |
| KeyApplications |
|
| BIOLOGicalInformation | |
|---|---|
| Source | recombinant,mouse,full-lengthMAPKinase2 |
| SpecificActivity | ForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme. |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Theactivationofthiskinaserequiresitsphosphorylationbyupstreamkinases.Uponactivation,thiskinasetranslocatestothenucleusofthestimulatedcells,whereitphosphorylatesnucleartargets.Twoalternativelysplicedtranscriptvariantsencodingthesameprotein,butdifferingintheUTRs,havebeenreportedforthisgene. |
| GeneSymbol |
|
| ProteinTarget | MAPK2 |
| PurificationMethod | Glutathioneagarose;Ni2+/NTAagarose |
| TargetSub-Family | CMGC |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P63085#Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.ThetheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsIBLeforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis. COFACTOR:Magnesium(Bysimilarity). DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain. |
| MolecularWeight | 67.8kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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