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Millipore/05-481 | Anti-phospho-MAP Kinase1/2 Antibody/05-481/100 µg
Millipore/05-481 | Anti-phospho-MAP Kinase1/2 Antibody/05-481/100 µg
市场价：
¥6580.00
美元价：
3948.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 05-481 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-phospho-MAPKinase1/2Antibody |
| AlternateNames |
|
| BackgroundInformation | Theextracellularsignal-regulatedkinases1and2(ERK1andERK2),alsocalledp44andp42MAPkinases,aremembersoftheMitogenActivatedProteinKinase(MAPK)familyofproteinsfoundinalleukaryotes.Becausethe44kDaERK1andthe42kDaERK2arehighlyhomologousandbothfunctioninthesameproteinkinasecascade,thetwoproteinsareoftenreferredtocollectivelyasERK1/2orp44/p42MAPkinase.TheERK1/2signalingcascadehasbeenshowntobeacriticalregulatorofcelldifferentiation,cellphysiologyandneuronalfunction.AberrantcontrolofERK1/2activityhasbeenimplicatedinavarietyofpathologicalconditions,includingcancerandautoimmunediseases,andefficientstudymethodsareindemand.Extracellularsignal/mitogenactivatedproteinkinases(erk/MAPK)areagroupofproline-directedserine/threoninekinasesthatareactivatedbydualphosphorylationofconservedthreonineandtyrosineresidueswithinacharacteristicTXYpeptidemotif.Themitogen-activatedkinaseserk1(MAPK1)anderk2(MAPK2)acquirefullenzymaticactivityuponphosphorylationofboththreonineandtyrosineresidueswithinthesequencemotifTEY. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG1klyophilizedfrom1mL2xPBS/0.09%Na-azide/PEGandSucrose. Reconstitutewith1mLH2O(15min,RT)forafinalconcentrationof100μg/mL. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearatfromdateofreceipt. |
| Applications | |
|---|---|
| Application | Detectphospho-MAPKinase1/2usingthisAnti-phospho-MAPKinase1/2validatedforuseinWB,IP,ELISA&IC. |
| KeyApplications |
|
| ApplicationNotes | Immunoprecipitation:Use1-10μgperimmuno-precipitation. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedphosphopeptidecontainingthepTEpYmotifofErk1andErk2.Clone:12D4 |
| Clone | clone12D4 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Specificforphosphorylated(activated)Erk1andErk2.AntibodyrecognitionrequiresphosphorylationofboththethreonineandtyrosineresiduesofErk1/Erk2andwillnotinteractwiththenon-phosphorylatedformoftheproteins.DoesnotcrossreactwithactivatedSAPKinase1or2. |
| Isotype | IgG1κ |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actinasignalingcascadethatregulatesvariouscellularprocessessuchasproliferation,differentiation,andcellcycleprogressioninresponsetoavarietyofextracellularsignals.Thiskinaseisactivatedbyupstreamkinases,resultinginitstranslocationtothenucleuswhereitphosphorylatesnucleartargets.Alternativelysplicedtranscriptvariantsencodingdifferentproteinisoformshavebeendescribed.... |
| GeneSymbol |
|
| Modifications |
|
| PurificationMethod | Thiophilicadsoptionandsizeexclusionchromatography |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P27361#Involvedinboththeinitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactorssuchasELK-1.PhosphorylatesEIF4EBP1;requiredforinitiationoftranslation.Phosphorylatesmicrotubule-associatedprotein2(MAP2).PhosphorylatesSPZ1(Bysimilarity).Phosphorylatesheatshockfactorprotein4(HSF4). COFACTOR:Magnesium(Bysimilarity). SIZE:379aminoacids;43136Da SUBUNIT:InteractswithMORG1(Bysimilarity).BindstoHIV-1Nef.Thisinteractioninhibitsitskinaseactivity.InteractswithHSF4. DOMAIN:SwissProt:P27361TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-202andTyr-204,whichactivatestheenzyme. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain.... |
| MolecularWeight | 42-44kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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