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Millipore/04-797 | Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39/04-797/100 µL
Millipore/04-797 | Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39/04-797/100 µL
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¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-797 |
| Replaces | 05-797 |
| BrandFamily | Upstate |
| TradeName |
|
| Description | Anti-phospho-MAPKinase1/2(Erk1/2)(Thr185/Tyr187)Antibody,cloneAW39 |
| AlternateNames |
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| BackgroundInformation | Erk(Extracellularsignal-RelatedKinase)isafamilyoftwo,highlyhomologousproteinsdenotedasErk1(p44,MAPK3)andErk2(p42,MAPK1)thatbothfunctioninthesamepathway.ThetwoproteinsareoftenreferredtocollectivelyasERK1/2orp44/p42MAPkinase.TheErkpathwayisconsideredtheclassical,canonicalMAPK(Mitogen-ActivatedProteinKinase)signalingpathway.Itisanevolutionarilyconservedpathwaythatcontrolsandisacriticalregulatorthegrowthandsurvivalthroughthepromotionofcellproliferationandthepreventionofapoptosis.Erkisinvolvedinthecontrolofmanyfundamentalcellularprocessesincludingcellproliferation,survival,differentiation,apoptosis,motilityandmetabolism.Erkisactivatedbygrowthfactorstimulationofreceptortyrosinekinases(RTKs)and/orintegrinstimulation.ThisactivatestheRas-Raf-MEK,-ErkpathwaythatresultsinMEK,adualkinase(aSer/ThrandaTyrkinase),phosphorylationandactivationofERK1/2(p44/044)ontheTxYmotif(Thr202/Tyr204andThr185/Tyr187forErk1andErk2,respectively). |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Culturedsupernantantin0.05%sodiumazide |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. |
| Applications | |
|---|---|
| KeyApplications |
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| ApplicationNotes | WesternBlotAnalysis: A1:2,000-1:8,000dilutionofthislotdetectedphospho-MAPKinase1/2(Erk1/2)inRIPAlysatesfromNGFstimulatedPC-12cells(FigureA). Beadlyte®Phospho-SpecificityAssay: 1:500-1:4,000dilutionsofapreviouslotdetectedactivebutnotunactiveMAPKinase/Erk2conjugatedtoLuminex®microspheres.(FigureB). |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugated,syntheticpeptidecorrespondingtoaminoacidssurroundingthepTEpYmotifofhumanphospho-MAPKinase1/2(Erk1/2),inwhichtheThrandTyrresiduesarephosphorylated.Theimmunizingsequenceishighlyconservedamongspecies. |
| Clone | Aw39 |
| Concentration | Variable |
| Host | Rabbit |
| Specificity | RecognizesphosphorylatedMAPKinase1and2(Erk1&2). |
| Isotype | IgG |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Humanandrat.Predictedtocross-reactwithmanyspeciesbasedonsequencehomology. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Theactivationofthiskinaserequiresitsphosphorylationbyupstreamkinases.Uponactivation,thiskinasetranslocatestothenucleusofthestimulatedcells,whereitphosphorylatesnucleartargets.Twoalternativelysplicedtranscriptvariantsencodingthesameprotein,butdifferingintheUTRs,havebeenreportedforthisgene. |
| GeneSymbol |
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| Modifications |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P28482#Involvedinboththeinitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactorssuchasELK1.PhosphorylatesEIF4EBP1;requiredforinitiationoftranslation.Phosphorylatesmicrotubule-associatedprotein2(MAP2).PhosphorylatesSPZ1(Bysimilarity).Phosphorylatesheatshockfactorprotein4(HSF4). COFACTOR:Magnesium(Bysimilarity). SIZE:360aminoacids;41390Da SUBUNIT:InteractswithMORG1(Bysimilarity).BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithHSF4. DOMAIN:SwissProt:P28482TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain. |
| MolecularWeight | MAPK1(~44kDa)andMAPK2(~42kDa). |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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