Millipore/04-349 |抗MAP激酶2/Erk2抗体，克隆E460，兔单克隆/04-349/100µ；L
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 04-349 |
| Description | Anti-MAPKinase2/Erk2Antibody,cloneE460,rabbitmonoclonal |
| AlternateNames |
|
| BackgroundInformation | Extracellularsignalregulatedkinase2(ERK2)alsoknownasmaturationormitogenactivatedproteinkinase(MAPK)isinvolvedineukaryoticsignaltransduction(1).Activatedbyadualphosphorylationonthreonineandtyrosineresiduebythedual-specificitykinaseMKK1/2,ERK2p42translocatetothenucleusafterstimulation(2).Oncephosphorylated,ERK2canformdimerswithphosphorylatedandunphosphorylatedERK2partners.ItisalsoabletophosphorylatemultiplesubstratessuchasEGFR,c-PLA2,Elk1/TCFandc-Myc(3-5). |
| ProductInformation | |
|---|---|
| Format | Unpurified |
| Control |
|
| Presentation | RabbitMonoclonalinbuffercontainingglycerol,BSA,andsodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponfirstthaw,andpriortoremovingcap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20ºC.Avoidfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | SyntheticpeptidecorrespondingtotheC-terminusofhumanMAPKinase2/Erk2. |
| Epitope | C-terminus |
| Clone | E460 |
| Host | Rabbit |
| Isotype | IgG |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
|
| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.ThetheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsIBLeforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis.Thesubstratesincludetranscriptionfactors(suchasATF2,BCL6,ELK1,ERF,FOS,HSF4orSPZ1),cytoskeletalelements(suchasCANX,CTTN,GJA1,MAP2,MAPT,PXN,SORBS3orSTMN1),regulatorsofapoptosis(suchasBAD,BTG2,CASP9,DAPK1,IER3,MCL1orPPARG),regulatorsoftranslation(suchasEIF4EBP1)andavarietyofothersignaling-relatedmolecules(likeARHGEF2,FRS2orGRB10).Proteinkinases(suchasRAF1,RPS6KA1/RSK1,RPS6KA3/RSK2,RPS6KA2/RSK3,RPS6KA6/RSK4,SYK,MKNK1/MNK1,MKNK2/MNK2,RPS6KA5/MSK1,RPS6KA4/MSK2,MAPKAPK3orMAPKAPK5)andphosphatases(suchasDUSP1,DUSP4,DUSP6orDUSP16)areothersubstrateswhichenablethepropagationtheMAPK/ERKsignaltoadditionnalcytosolicandnucleartargets,therebyextendingthespecificityofthecascade. Actsasatranscriptionalrepressor.Bindstoa[GC]AAA[GC]consensussequence.Represstheexpressionofinterferongamma-inducedgenes.SeemstobindtothepromoterofCCL5,DMP1,IFIH1,IFITM1,IRF7,IRF9,LAMP3,OAS1,OAS2,OAS3andSTAT1.Transcriptionalactivityisindependentofkinaseactivity. CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein. COFACTOR:MagnesiumBysimilarity. ENZYMEREGULATION:PhosphorylatedbyMAP2K1/MEK1andMAP2K2/MEK2onThr-185andTyr-187inresponsetoexternalstimulilikeinsulinorNGF.Bothphosphorylationsarerequiredforactivity.Thisphosphorylationcausesdramaticconformationalchanges,whichenablefullactivationandinteractionofMAPK1/ERK2withitssubstrates.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DephospphorylatedandinactivatedbyDUSP3,DUSP6andDUSP9.Inactivatedbypyrimidylpyrroleinhibitors. SUBUNITSTRUCTURE:Bindsbothupstreamactivatorsanddownstreamsubstratesinmultimolecularcomplexes.BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithADAM15,ARHGEF2,ARRB2,DAPK1(viadeathdomain),HSF4,IER3,IPO7,DUSP6,NISCH,SGK1,andisoform1ofNEK2.Interacts(phosphorylatedform)withCAV2("Tyr-19"-phosphorylatedform);theinteraction,promotedbyinsulin,leadstonuclearlocationandMAPK1activation.InteractswithMORG1,PEA15andMKNK2Bysimilarity.MKNK2isoform1bindingpreventsfromdephosphorylationandinactivationBysimilarity. SUBCELLULARLOCATION:Nucleus.Cytoplasm›Cytoskeleton›centrosome.Cytoplasm.Note:PEA15-bindingandphosphorylatedDAPK1promoteitscytoplasmicretention.PhosphorylationatSer-246andSer-248aswellasautophosphorylationatThr-190promotenuclearlocalization. DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:PhosphorylateduponKITandFLT3signalingBysimilarity.DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme.UndergoesregulatoryphosphorylationonadditionalresiduessuchasSer-246andSer-248inthekinaseinsertdomain(KID)Thesephosphorylations,whichareprobablymediatedbymorethanonekinase,areimportantforbindingofMAPK1/ERK2toimportin-7(IPO7)anditsnucleartranslocation.Inaddition,autophosphorylationofThr-190wasshowntoaffectthesubcellularlocalizationofMAPK1/ERK2aswell.Ligand-activatedALKinducestyrosinephosphorylation.DephosphorylatedbyPTPRJatTyr-187.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DUSP3andDUSP6dephosphorylatespecificallyMAPK1/ERK2andMAPK3/ERK1whereasDUSP9dephosphorylatesabroaderrangeofMAPKs. SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily. Contains1proteinkinasedomain. SEQUENCECAUTION:ThesequenceCAA77753.1differsfromthatshown.Reason:Erroneousinitiation.TranslationN-terminallyextended. |
| MolecularWeight | ~42kDaobserved |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
