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当前位置: 首页 > 产品中心 > Functional_antibody > 微孔/506123 |抗p38 MAP激酶(341-360)兔pAb/506123/
商品详细微孔/506123 |抗p38 MAP激酶(341-360)兔pAb/506123/
微孔/506123 |抗p38 MAP激酶(341-360)兔pAb/506123/
微孔/506123 |抗p38 MAP激酶(341-360)兔pAb/506123/
商品编号: 506123
品牌: 密理博
市场价: ¥7400.00
美元价: 4440.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
OverviewRecognizesthe~38kDap38MAPKprotein.
  • AntibodyTargetGeneSymbol:MAPK14
  • TargetSynonym:CRK1,CSBP,CSBP1,CSBP2,CSPB1,EXIP,Hog,MAPKp38,MGC102436,MGC105413,MXI2,P38,P38KINASE,P38MapKinase,p38Mapkalpha,P38-ALPHA,p38-RK,p38/Hog1,p38/Mpk2,P38/RK,p38a,p38Hog,p38MAPK,PRKM14,PRKM15,RK,SAPK2A
  • EntrezGeneName:mitogen-activatedproteinkinase14
  • HuEntrezID:1432
  • MuEntrezID:26416
  • RatEntrezID:81649
  • CatalogueNumber506123
    BrandFamilyCalbiochem®
    ApplicationData
    Detectionofp38MAPkinasebyimmunoblotting.Samples:C6cells(lanes1and2)treated(lane1)oruntreated(lane2)withanisomycinandNIH/3T3cells(lanes3and4)treated(lane3)oruntreated(lane4)withUV.Primaryantibody:Anti-p38MAPKinase(341-360)RabbitpAb(Cat.No.506123)(1:1000).Detection:chemiluminescence.
    ProductInformation
    FormLiquid
    FormulationIn150mMNaCl,10mMHEPES,50%glycerol,0.01%BSA,pH7.5.
    PreservativeNone
    StorageandShippingInformation
    ShipCodeBlueIceOnly
    ToxicityStandardHandling
    Storage-20°C
    Avoidfreeze/thawAvoidfreeze/thaw
    DonotfreezeOktofreeze
    SpecialInstructionsFollowinginitialthaw,aliquotandfreeze(-20°C).
    Applications
    KeyApplicationsFlowCytometry
    Immunoblotting(WesternBlotting)
    ParaffinSections
    ApplicationNotesFlowCytometry(1:25)
    Immunoblotting(1:1000)
    ParaffinSections(1:50,heatpretreatmentrequired,seecomments)
    ApplicationCommentsPretreatparaffinsectionsbyheatingtissuein10mMcitratebuffer,pH6.0for1minathighpowerfollowedby9minatmediumpower;keeptheslidesfullyimmersedandmaintainthetemperatureatorjustbelowboiling;cooltheslidesfor20minatroomtemperaturepriortostaining.Variablesassociatedwithassayconditionswilldictatetheproperworkingdilution.

    RecommendedProtocolforImmunoblotting

    SolutionsandReagents
    •TransferBuffer:25mMTrisbase,0.2Mglycine,20%methanol,pH8.5.
    •SDSSampleBuffer:62.5mMTris-HCl,pH6.8,2%SDS,10%glycerol,50mMDTT,0.1%bromophenolblue.
    •10XTBS(Tris-bufferedsaline):Toprepare1liter,24.2gTrisbase,80gNaCl,adjustpHto7.6withHCl.Dilute1:10foruse.
    •BlockingBuffer:1XTBS,0.1%Tween®-20detergentwith5%non-fatdrymilk.
    •PrimaryAntibodyDilutionBuffer:1XTBS,0.1%Tween®-20detergentwith5%BSA
    •WashBuffer(TBST):1XTBS,0.1%Tween®-20detergent

    BlottingMembrane
    NitrocelluloseorPVDFmembranesmaybeused.

    ProteinBlotting
    1.Lysecellsbyadding100mlSDSSampleBufferandimmediatelyscrapethecellsofftheplateandtransfertheextracttoamicrofugetube.Keeponice.
    2.Sonicatefor2stoshearDNAandreducesampleviscosity.
    3.Heatsampleto95-100°Cfor5min.Coolonice.
    4.Microcentrifugefor5min.
    5.Load20mlontoSDS-PAGEgel(10cmx10cm).
    6.Electrotransfertonitrocellulosemembrane.

    Ascontrols,werecommendusing15mlofphosphorylatedandnonphosphorylatedC-6gliomacellextracts.

    MembraneBlocking,GelandAntibodyIncubations
    1.Aftertransfer,washmembranewith25mlTBSfor5minatroomtemperature.
    2.Incubatemembranein25mlofBlockingBufferfor1-3hatroomtemperatureorovernightat4°C.
    3.Wash3timesfor5mineachwith15mlTBST.
    4.Incubatemembraneandprimaryantibody(attheappropriatedilution)in10mlPrimaryAntibodyDilutionBufferwithgentleagitationovernightat4°C.
    5.Wash3timesfor5mineachwith15mlTBST.
    6.Incubatemembranewithconjugatedsecondaryantibodyattheappropriatedilutionin10mlBlockingBufferwithgentleagitationfor1hatroomtemperature.
    7.Washmembraneasinstep5.

    DetectionofProteins
    Chemiluminescence.
    BIOLOGicalInformation
    Immunogenasyntheticpeptide(TYDEYISFVPPPLDQEEMES)correspondingtoaminoacids341-360ofhumanp38MAPkinase
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    SpeciesReactivity
    • Human
    • Mouse
    • Rat
    AntibodyTypePolyclonalAntibody
    PhysicochemicalInformation
    Dimensions
    MaterialsInformation
    MaterialsInformation
    品牌介绍
    密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。