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Millipore/MAB4078 | Anti-PCNA Antibody, clone 131-11912/MAB4078/100 µL
Millipore/MAB4078 | Anti-PCNA Antibody, clone 131-11912/MAB4078/100 µL
市场价：
¥5960.00
美元价：
3576.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB4078 |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-PCNAAntibody,clone131-11912 |
| AlternateNames |
|
| ProductInformation | |
|---|---|
| Format | Ascites |
| Presentation | Ascitesfluidcontainingnopreservatives |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfrozenat-20°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles. |
| Applications | |
|---|---|
| Application | Anti-PCNAAntibody,clone131-11912isamousemonoclonalantibodyfordetectionofPCNAalsoknownasProliferatingCellNuclearAntigen,DNAPolymerasedeltaProcessivityFactor&hasbeenvalidatedinIF,ICC,IHC(P). |
| KeyApplications |
|
| ApplicationNotes | Immunohistochemistryonformalinfixed,deparaffinizedtissuesections Frozentissuesections. Smears. Cytospins. *DonotuseSeruminblockingsteps. Alsoseehttp://ajp.amjpathol.org/cgi/content/abstract/141/6/1453forimportantreactivityinformationon19A2inparaffinfixedtissues. Finalworkingdilutionsmustbedeterminedbyenduser. IMMUNOHISTOCHEMICALPROCEDUREFORMAB4078 ThefollowinghistochemicalimmunoperoxidaseprotocolhasbeenoptimizedforusewithMAB4078. METHODOLOGY:ThismethodisusedwithstandardstreptavidinorABCimmunoperoxidasemethodsusingaDABsubstraterunatroomtemperaturewithparaffinorfrozensections,smearsorcytospins. NOTE:Optimalfixationtimeforparaffinprocessedtissuefixedin10%NeutralBufferedFormalinis12-14hours. REQUIREDSUPPLEMENTALMATERIAL: 1.3%HydrogenPeroxide:commerciallyavailable. 2.PBS 3.EthylAlcohol:histologygradeorequivalent 4.Methanol:histologygradeorequivalent 5.Acetone:histologygradeorequivalent 6.Xylene(histologygrade)orXyleneSubstitute(Hemo-D) 7.MountingMedia:Krystalon(Harleco)orequivalent 8.Gill#1Hematoxylin:Sigma#GHS-1-32orequivalent 9.GoatAnti-MouseBiotin 10.StreptavidinHRP(optionalreagent) 11.3,3"Diaminobenzidinetetrahydrochloride(DAB):Sigma#D-5637orequivalent 12.ABC:VectorKit#PK4000orequivalent REAGENTPREPARATION: 1.Primaryantibody:PrepareastocksolutionofantibodyinPBS 2.GoatAnti-MouseBiotin:Approximatedilutionof1:100inPBS(10mg/test) 3.Streptavidin:5mLstocksolutionand995mLPBS(1/200dilution).Letsit15minutesbeforeuse(yieldasavolumesufficientfor~20slides)ORifusingABCkitfromVectorfollowasinstructed. 4.DAB:Reconstitute1.5mgofpowderwith100mLofPBS.Activate15minutespriortousebyadding100mL3%H2O2(yieldsavolumesufficientfor~20slides).WARNING:DABisapossIBLecarcinogen.Avoidcontactwithskin. 5.PBS:Usingagraduatedcylinder,reconstitutewith500mLofdistilledwater. 6.AlcoholBaths:Alldilutionsarealcoholsmadewithdistilledwater. PROCEDURE: PARAFFINSECTIONS (cutat4-5microns,10%NeutralBufferedFormalinfixed): 1.Placeslidesin62°Covenfor15minutes. 2.PlaceslidesinHemo-D,orxylene,for15minutes. 3.De-paraffinizeslidesthroughbathsof:A)xylene/100%ethanol1:1(orHemo-D/100%ethanol1:1),B)100%ethylalcoholx2,C)95%ethylalcohol,D)90%ethylalcohol,E)80%ethylalcohol,F)70%ethylalcoholx2(2minuteseachbath). 4.Placeslidesinrunningcooltapwaterfor5minutes. 5.Shakeoffexcesswater.Placeslidesonincubationracksheldatroomtemperature.CirclesectionswithPAPpen(Newcomer,Inc.)ordiamondpentoisolatesection. 6.Floodsectionswithprimaryantibodyandincubatefor35-40minutes. 7.WipeoffexcessPBS.FloodsectionswithGoatAnti-MouseBiotinreagentandincubatefor35-40minutes. 8.WashwithPBS3-5min 9.WashagainwithPBS3-5min. 10.WipeoffexcessPBS.Floodsectionswithstreptavidin-HRPandincubatefor10minutesORfloodsectionswithABCandincubatefor35-40minutes. 11.WashwithPBS3-5min. 12.WipeoffexcessPBS.FloodsectionswithDABandincubatefor6-8minutes. 13.Placeslidesincoolrunningtapwaterfor5minutes. 14.PlaceslidesinGills#1hematoxylinfor90seconds. 15.Placeslidesincoolrunningtapwaterfor5minutes. 16.DehydrateslidesthroughbathsofA)70%ethylalcohol,B)80%ethylalcohol,C)90%ethylalcohol,D)95%ethylalcoholx2,E)100%ethylalcoholx2,F)xylene/100%ethanol1:1(orHemo-D/100%ethanol1:1)x2,G)xyleneorHemo-Dx2(2minuteseachbath. 17.Mountslidesusingmountingmedia. FROZENSECTIONS(cutat8microns):Pullsectionsfromfreezer(orcutfresh),airdryatroomtemperature(orunderafanfor15minutes),fixedin4ºCacetonefor4minutes,airdryfor30minutesatroomtemperature(orunderafanfor15minutes),thenfollowaboveprocedurebeginningat#5. SMEARS:Methanolfixfor30minutesat4ºC,thenfollowaboveprocedurebeginningat#5. CYTOSPINS:CelllinesandothercellpopulationspreparedbyresUSPending,ineachwell,~40,000cellsusing500mL1%NormalGoatSerumperwell.Centrifugefor6minutesat1200RPM.Airdryfor30minutesatroomtemperature(orunderafanfor15minutes).Iftumorcellsarebeingscreened,fixinRTacetonefor2-3minutes.Ifperipheralbloodprepsarebeingscreened,fixin100%methanolfor20minutes.Airdrytheslidesfor30minutesatroomtemperature(or15minutesunderafan)andbeginat#5above. NOTE:WhenstainingusingthePCNAantibodiesastheprimaryDONOTuseNormalGoatSerumoranyotherserumblockingstep. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | PurifiedPCNA |
| Clone | 131-11912 |
| Host | Mouse |
| Specificity | SpecificforPCNAa36kDaproliferationassociatedantigen. |
| Isotype | IgM |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisfoundinthenucleusandisacofactorofDNApolymerasedelta.TheencodedproteinactsasahomotrimerandhelpsincreasetheprocessivityofleADIngstrandsynthesisduringDNAreplication.InresponsetoDNAdamage,thisproteinisubiquitinatedandisinvolvedintheRAD6-dependentDNArepairpathway.Twotranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.Pseudogenesofthisgenehavebeendescribedonchromosome4andontheXchromosome. |
| GeneSymbol |
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12004#ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleadingstrand. SIZE:261aminoacids;28769Da SUBUNIT:Homotrimer.InteractswithKCTD10(Bysimilarity).Formsacomplexwithactivator1heteropentamerinthepresenceofATP.InteractswithPOLH,POLK,DNMT1,ERCC5/XPG,FEN1,CDC6,APEX2andPOLDIP2.InteractswithEXO1andSHPRH. SUBCELLULARLOCATION:Nucleus. PTM:Uponmethylmethanesulfonate-inducedDNAdamage,mono-ubiquitinatedbytheUBE2B-RAD18complexonLys-164.Thisinducesnon-canonicalpoly-ubiquitinationonLys-164through"Lys-63"linkageofubiquitinmoietiesbytheE2complexUBE2N-UBE2V2andtheE3ligaseSHPRH,whihisrequiredforDNArepair. DISEASE:SwissProt:P12004#Antibodiesarepresentinserafrompatientswithsystemiclupuserythematosus. SIMILARITY:SwissProt:P12004##BelongstothePCNAfamily. |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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