Millipore/CBL407 |抗PCNA抗体，克隆PC10/CBL407/100和micro；G
市场价：
¥5340.00
美元价：
3204.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | CBL407 |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | Anti-PCNAAntibody,clonePC10 |
| AlternateNames |
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| BackgroundInformation | Proliferatingcellnuclearantigen(PCNA)isa36kDamoleculethatishighlyconservedbetweenspecies.PCNAwasfirstidentifiedastheantigenforasubpopulationofautoantibodiesinpatientswithsystemiclupuserythematosus(Miyachi,1978;Takasaki,1984;Ogata,1987).IthassincebeendeterminedthatPCNAservesasaco-factorforDNApolymerasedeltainS-phaseaswellasduringDNAsynthesisassociatedwithmechanismsinvolvedinDNAdamagerepair(Tan,1987;Bravo,1987).ThetemporalspecificityofPCNAexpressionmakesitanidealMarkerforcellproliferation.PCNAbeginstoaccumulateduringtheG1phaseofthecellcycle,ismostabundantduringtheSphase,anddeclinesduringtheG2/Mphase(Kurki,1988).Sincethehalf-lifeofPCNAexceeds20hours,itmaybepossIBLetodetecttheproteininnon-cyclingcells. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Themonoclonalispresentedataconcentrationof100μg/1mLinphosphatebufferedsalinecontaining10mMsodiumazideand1mg/mlbovineserumalbumin.Werecommendthateachlaboratorydetermineanoptimumworkingtitreforuseinitsparticularapplication. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Forusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C.Avoidrepeatedfreeze/thawing. |
| Applications | |
|---|---|
| Application | Anti-PCNAAntibody,clonePC10isamousemonoclonalantibodyfordetectionofPCNAalsoknownasProliferatingCellNuclearAntigen,DNAPolymerasedeltaProcessivityFactor&hasbeenvalidatedinELISA,FC,IP,WB,IHC(P). |
| KeyApplications |
|
| ApplicationNotes | Westernblot:1:250-1:1000.Nuclearextractsarepreferredoverwholecellproteinextracts. Immunohistochemistry:1:20-1:200.Recommendedforparaffinembeddedtissuesectionsonly.Maybeusedonmaterialfixedinawiderangeoffixativesincludingformalin(bufferedandunbuffered),methacarnandBouin"sreagent.ThetimeoffixationcanmarkedlyaffecttheintensityofPCNAimmunoreactivity.Stainingisreduced(andmaybeabolished)ifsectionsarebakedontoglassslides.Air-dryingovernightonpoly-L-lysinecoatedslidesisrecommended.60minuteincubationat25°CwithstandardABCtechniqueisrecommended. Immunoprecipitation IndirectFlowCytometry Optimalworkingdilutionsmustbedeterminedbytheenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | RatPCNAmadeintheproteinAvectorpR1T2T. |
| Clone | PC10 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | ClonePC10recognizesPCNAfromallvertebrateandinsectspeciestestedandhasbeenusedtoidentifytransformedcells(Kurki,1988),proliferatingcellsinsolidtumors(Smetana,1983),andblastcellsinleukemiapatients(Takasaki,1984).ImmunohistochemistrywithclonePC10hasbeenusedtostudytheexpressionofPCNAinparaffinsectionsofnormaltissuesandlymphoidneoplasms(Hall,1990).Inproliferatingcells,PCNAstainingpatternispredominantlynuclear.Bywesternblot,theantibodydetectsapolypeptidemigratingat36kDawithanisoelectricpointof4.8. |
| Isotype | IgG2a |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisfoundinthenucleusandisacofactorofDNApolymerasedelta.TheencodedproteinactsasahomotrimerandhelpsincreasetheprocessivityofleADIngstrandsynthesisduringDNAreplication.InresponsetoDNAdamage,thisproteinisubiquitinatedandisinvolvedintheRAD6-dependentDNArepairpathway.Twotranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.Pseudogenesofthisgenehavebeendescribedonchromosome4andontheXchromosome. |
| GeneSymbol |
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| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12004#ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleadingstrand. SIZE:261aminoacids;28769Da SUBUNIT:Homotrimer.InteractswithKCTD10(Bysimilarity).Formsacomplexwithactivator1heteropentamerinthepresenceofATP.InteractswithPOLH,POLK,DNMT1,ERCC5/XPG,FEN1,CDC6,APEX2andPOLDIP2.InteractswithEXO1andSHPRH. SUBCELLULARLOCATION:Nucleus. PTM:Uponmethylmethanesulfonate-inducedDNAdamage,mono-ubiquitinatedbytheUBE2B-RAD18complexonLys-164.Thisinducesnon-canonicalpoly-ubiquitinationonLys-164through"Lys-63"linkageofubiquitinmoietiesbytheE2complexUBE2N-UBE2V2andtheE3ligaseSHPRH,whihisrequiredforDNArepair. DISEASE:SwissProt:P12004#Antibodiesarepresentinserafrompatientswithsystemiclupuserythematosus. SIMILARITY:SwissProt:P12004##BelongstothePCNAfamily. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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