密理博/07-2162 |抗PCNA抗体/07-2162/100µ；G
市场价：
¥5540.00
美元价：
3324.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | 07-2162 |
| Description | Anti-PCNAAntibody |
| AlternateNames |
|
| BackgroundInformation | Proliferatingcellnuclearantigen(PCNA)belongstotheDNAslidingclampfamilyandiscomprisedofthreeidenticalmonomersformingaheterodimer.Duetoitsuniquestructure,PCNAisabletoincreasetheprocessivityofDNApolymerasedeltabyusingitsouterdomainstobeinvolvedinprotein-proteininteractionwhilestilltetheredtoandfreelyslidingalongtheDNA.PCNAisacriticalregulatorycomponentintheonsetofleADIngstrandDNAreplicationandtheterminationoflaggingstrandsynthesis.StudieshavealsoindicatedaroleforPCNAintherecruitmentandregulationofMMRpathwayrepairproteins.PCNAisalsoabletointeractwithawidevarietyofcellcycleproteinsandservestocoordinateseveralproteinsparticipatinginDNAprocesses,suchasapoptosis. |
| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | GST-taggedrecombinantproteincorrespondingtohumanPCNA. |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Demonstratedtoreactwithhuman,mouse,andrat.Predictedtoreactwithchimpanzeebasedon100%sequencehomology.Otherhomologies:Bovine,rabbit,canine,andpanda(99%sequencehomology). |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisfoundinthenucleusandisacofactorofDNApolymerasedelta.TheencodedproteinactsasahomotrimerandhelpsincreasetheprocessivityofleadingstrandsynthesisduringDNAreplication.InresponsetoDNAdamage,thisproteinisubiquitinatedandisinvolvedintheRAD6-dependentDNArepairpathway.Twotranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.Pseudogenesofthisgenehavebeendescribedonchromosome4andontheXchromosome.[providedbyRefSeq]. |
| GeneSymbol |
|
| PurificationMethod | ProteinA |
| UniProtNumber | |
| UniProtSummary | FUNCTION:ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleadingstrand. SUBUNITSTRUCTURE:Homotrimer(Bysimilarity).Formsacomplexwithactivator1heteropentamerinthepresenceofATP.InteractswithEXO1,POLH,POLK,DNMT1,ERCC5,FEN1,CDC6,APEX2andPOLDIP2.FormsaternarycomplexwithDNTTIP2andcorehistone.InteractswithKCTD10andPPP1R15A(Bysimilarity).InteractswithPOLD1,POLD3andPOLD4.InteractswithBAZ1B;theinteractionisdirect.InteractswithHLTFandSHPRH.InteractswithNUDT15.InteractionisdisruptedinresponsetoUVirradiationandacetylation.InteractswithCDKN1A/p21(CIP1)andCDT1;interactsviatheirPIP-boxwhichalsorecruitstheDCX(DTL)complex. SUBCELLULARLOCATION:Nucleus. PTM:Uponmethylmethanesulfonate-inducedDNAdamage,mono-ubiquitinatedbytheUBE2B-RAD18complexonLys-164.Thisinducesnon-canonicalpolyubiquitinationonLys-164through"Lys-63"linkageofubiquitinmoietiesbytheE2complexUBE2N-UBE2V2andtheE3ligases,HLTF,RNF8andSHPRH,whichisrequiredforDNArepair."Lys-63"polyubiquitinationpreventsgenomicinstABIlityonDNAdamage.MonoubiquitinationatLys-164alsotakesplaceinundamagedproliferatingcells,andismediatedbytheDCX(DTL)complex,leadingtoenhancePCNA-dependenttranslesionDNAsynthesis. AcetylatedinresponsetoUVirradiation.AcetylationdisruptsinteractionwithNUDT15andpromotesdegradation. MISCELLANEOUS:AntibodiesagainstPCNAarepresentinserafrompatientswithsystemiclupuserythematosus. SEQUENCESIMILARITIES:BelongstothePCNAfamily. |
| MolecularWeight | ~35kDaobserved.PCNAhasapredictedmolecularweightof29kDa,however,itcanbemonoubiquitinated,resultingina~35kDaband. |
| PhysicochemicalInformation |
|---|
| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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