密理博/HTS055M |化学筛™ 重组人CXCR5趋化因子受体/HTS055M/200细胞膜的制备
市场价：
¥11920.00
美元价：
7152.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | HTS055M |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | ChemiSCREEN™MembranePreparationRecombinantHumanCXCR5ChemokineReceptor |
| Overview | HumanCXCR5 |
| BackgroundInformation | CXCR5(alsoknownasBLR1)isaGPCRthatbindsselectivelytothechemokineCXCL13(alsoknownasBLCandBCA-1)tomediateimmunesystemdevelopmentandfunction(Försteretal.,1996;Gunnetal.,1998).CXCR5cooperateswithCCR7todeterminefunctionalorganizationoflymphoidorganssuchaslymphnodesandPeyer’spatches(Ohletal.,2003).Inaddition,CXCR5mediatesmigrationofBcellsintothefolliclesofthesplenicwhitepulp,thuspermittingantigendepositiononfolliculardendriticcells(Försteretal.,1996;Cinamonetal.,2008).Patientswithrheumatoidarthritis(RA)displayedupregulatedexpressionofCXCR5toagreaterextentthanotherchemokinereceptors,andCXCR5-nullmicesubjectedtoantigen-inducedarthritisdisplayreducedjointdestruction(Schmutzetal,2004;Wengneretal,2007).Therefore,CXCR5representsapotentialtargetfortreatmentofrheumatoidarthritis.Millipore"sCXCR5MembranePreparationforGTPγSAssayisacrudemembranepreparationmadefromtheproprietarystablerecombinantcelllinethatisalsousedforMillipore’sCXCR5CalciumOptimizedCellLine(HTS055C).ThisCXCR5MembranePreparationhasbeenoptimizedforlowbasalactivityinGTPγSbindingassays;thusitisanidealHTStoolforscreeningofcompoundsforagoNISTandantagonistactivities.ThemembranepreparationsexhibitsEC50of1.8nMforCXCL13inaGTPγSbindingassay. |
| ProductInformation | |
|---|---|
| Format | Membranes |
| Presentation | Liquidinpackagingbuffer:50mMTrispH7.4,10%glyceroland1%BSAwithnopreservatives. Packagingmethod:Membraneproteinwasadjustedtotheindicatedconcentrationinpackagingbuffer,rapidlyfrozen,andstoredat-80°C. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Storeat–70°C.Productisstableforatleast6monthsfromthedateofreceiptwhenstoredasdirected.Donotfreezeandthaw. |
| Applications | |
|---|---|
| Application | HumanCXCR5GPCRmembranepreparationforRADIoligandbindingAssays>PγSbinding. |
| KeyApplications |
|
| ApplicationNotes | RadioligandbindingassayandGTPγSbinding |
| BIOLOGicalInformation | |
|---|---|
| EntrezGeneNumber | |
| GeneSymbol |
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| GPCRClass | A |
| HostCells | Chem-5 |
| ProteinTarget | CXCR5 |
| TargetSub-Family | Chemokine |
| UniProtNumber | |
| IncubationConditions | MembranesarepermeABIlizedbyadditionofsaponintoanequalconcentrationbymass,thenmixedwith[35S]-GTPγS(finalconcentrationof0.3nM)in20mMHEPES,pH7.4/100mMNaCl/10mMMgCl2/0.5μMGDPinanonbinding96-wellplate.UnlabeledCXCL13wasaddedtothefinalconcentrationindicatedinFigure1(finalvolume100μL),andincubatedfor30minat30°C.ThebindingreactionistransferredtoanFBfilterplate(MilliporeMAHFB1H)previouslyprewettedwithwater.Theplateiswashed3times(1mLperwellperwash)withcold10mMsodiumphosphate,pH7.4,thendriedandcounted. Onepackagecontainsenoughmembranesforatleast200assays(units),whereoneunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificCXCL13-stimulated[35S]-GTPγSbinding. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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