微孔/HTS002M |化学筛™ CXCR2膜制备/HTS002M/200分析
市场价：
¥11920.00
美元价：
7152.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | HTS002M |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | ChemiScreen™CXCR2MembranePreparation |
| Overview | TRANSFECTION:Full-lengthhumanCXCR2CDNA(AccessionNumber:M73969) |
| BackgroundInformation | CXCR2isa7-TMG-proteincoupledreceptorthatbindstothechemokinesGROalpha,GRObeta,GROgamma,IL-8,ENA-78,NAP-2andGCP-2(OlsonandLey,2002).Neutrophils,mastcellsandmicrovascularendothelialcellsexpressCXCR2,andinteractionsofCXCR2withitsligandspromoteschemotaxisofthesecelltypes(Heidemannetal.,2003;Nilssonetal.,1999;Whiteetal.,1998).StudieswithmicelackingCXCR2indicatethatCXCR2promotesgrowthofprimarytumorsandsecondarymetastases(Keaneetal.,2004),andplaysanessentialroleinhyperoxia-inducedlunginjury(Sueetal.,2004).Inaddition,cytomegalovirusencodesaCXCR2-bindingchemokine,vCXC-1,thatpromotesneutrophilmigrationtoinfectedcells(Penfoldetal.,1999).Chemicon"sCXCR2MembranePrepsareidealtoolsforscreeningforantagoNISTsofinteractionsbetweenCXCR2anditsligands.RADIolabeledIL-8bindstotheCXCR2membraneswithaKdof0.075nM.With0.1nM125I-labeledIL-8,a5-foldwindowofspecificbindingisobtained. |
| ProductInformation | |
|---|---|
| Format | Membranes |
| Presentation | Membraneswereadjustedto0.5mgprotein/mlin50mMTrispH7.4,10%glyceroland1%BSA,rapidlyfrozen,andstoredat-80°C.Onevialtypicallycontains1000units,whereaunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificbindingof125I-labeledIL-8(0.1nMinputconcentration). |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfrozenat-70°Cforupto2years.Donotfreezeandthaw. |
| Applications | |
|---|---|
| Application | HumanCXCR2/IL-8bGPCRmembranepreparationforRadioligandbindingAssays. |
| KeyApplications |
|
| ApplicationNotes | Radioligandbindingassay. |
| BIOLOGicalInformation | |
|---|---|
| Concentration | 0.5mg/mL |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Thisreceptoralsobindstochemokine(C-X-Cmotif)ligand1(CXCL1/MGSA),aproteinwithmelanomagrowthstimulatingactivity,andhasbeenshowntobeamajorcomponentrequiredforserum-dependentmelanomacellgrowth.Thisreceptormediatesneutrophilmigrationtositesofinflammation.TheangiogeniceffectsofIL8inintestinalmicrovascularendothelialcellsarefoundtobemediatedbythisreceptor.Knockoutstudiesinmicesuggestedthatthisreceptorcontrolsthepositioningofoligodendrocyteprecursorsindevelopingspinalcordbyarrestingtheirmigration.Thisgene,IL8RA,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36. |
| GeneSymbol |
|
| GPCRClass | A |
| HostCells | Chem-1 |
| ProteinTarget | CXCR2/IL-8b |
| TargetSub-Family | Chemokine |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P25025#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoGRO/MGSAandNAP-2alsowithahighaffinity. SIZE:360aminoacids;40759Da SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein. PTM:Phosphorylateduponligandbinding;whichisrequiredfordesensitization. SIMILARITY:SwissProt:P25025##BelongstotheG-proteincoupledreceptor1family. |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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