密理博/HTS001M |化学筛™ 重组人CXCR1趋化因子受体/HTS001M/200细胞膜的制备
市场价：
¥11920.00
美元价：
7152.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | HTS001M |
| BrandFamily | Chemicon® |
| TradeName |
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| Description | ChemiSCREEN™MembranePreparationRecombinantHumanCXCR1ChemokineReceptor |
| Overview | :Full-lengthhumanCXCR1CDNA |
| BackgroundInformation | CXCR1(alsoknownasIL-8RA)isaGi/o-coupledGPCRexpressedonneutrophils,CD8(+)Tcells,andintestinalepithelialcells(Murphy,1997;Sturmetal.,2005;Takataetal.,2004).CXCR1bindsspecificallytothechemokineinterleukin-8(IL-8),whereasarelatedreceptor,CXCR2,bindspromiscuouslytoIL-8andseveralotherchemokines.CXCR1hasbeenproposedtobetheprimaryregulatorofneutrophilresponsetoIL-8insepsis(Cummingsetal.,1999).AntagoNISTsofCXCR1andCXCR2havebeendevelopedanddisplayefficacyinanimalmodelsofreperfusioninjury(Bertini,etal.,2004;Kaneideretal.,2005).Millipore"sCXCR1membranepreparationsarecrudemembranepreparationsmadefromourproprietarystablerecombinantcelllinestoensurehigh-levelofGPCRsurfaceexpression;thus,theyareidealHTStoolsforscreeningofantagonistsofCXCR1interactionwithIL-8.ThemembranepreparationsexhibitaKdof0.2nMfor[125I]-IL-8.With5μg/wellCXCR1MembranePrepand0.1nM[125I]-IL-8,agreaterthan5-foldsignal-to-backgroundratioisobtained. |
| ProductInformation | |
|---|---|
| Format | Membranes |
| Presentation | Liquidinpackagingbuffer:50mMTrispH7.4,10%glyceroland1%BSAnopreservatives.Packagingmethod:Membranesproteinwereadjustedtotheindicatedconcentrationinpackagingbuffer,rapidlyfrozen,andstoredat-80°C. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Maintainfrozenat-70°Cforupto2years.Donotfreezeandthaw. |
| Applications | |
|---|---|
| Application | HumanCXCR1/IL-8aGPCRmembranepreparationforRADIoligandbindingAssays>PgammaSbinding. |
| KeyApplications |
|
| ApplicationNotes | Radioligandbindingassay,andGTPgammaSbinding. |
| BIOLOGicalInformation | |
|---|---|
| Concentration | 1mg/mL |
| EntrezGeneNumber | |
| EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Knockoutstudiesinmicesuggestedthatthisproteininhibitsembryonicoligodendrocyteprecursormigrationindevelopingspinalcord.Thisgene,IL8RB,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36. |
| GeneSymbol |
|
| GPCRClass | A |
| HostCells | Chem-1 |
| ProteinTarget | CXCR1/IL-8a |
| TargetSub-Family | Chemokine |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P25024#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoMGSA(GRO)withalowaffinity. SIZE:350aminoacids;39791Da SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein. SIMILARITY:SwissProt:P25024##BelongstotheG-proteincoupledreceptor1family. |
| IncubationConditions | RECOMMENDEDASSAYCONDITIONS:Membranesaremixedwithradioactiveligandandunlabeledcompetitor(seeFigures1and2forconcentrationstested)inbindingbufferinanonbinding96-wellplate,andincubatedfor1-2h.Priortofiltration,anFC96-wellharvestplate(Milliporecat.#MAHFC1H)iscoatedwith0.33%polyethyleneiminefor30min,thenwashedwith50mMHEPES,pH7.4,0.5%BSA.Bindingreactionistransferredtothefilterplate,andwashed3times(1mLperwellperwash)withWashBuffer.Theplateisdriedandcounted. Bindingbuffer:50mMHepes,pH7.4,5mMMgCl2,1mMCaCl2,0.2%BSA,filteredandstoredat4°C Radioligand:[125I]Interleukin-8(PerkinElmer#NEX277) WashBuffer:50mMHepes,pH7.4,500mMNaCl,0.1%BSA,filteredandstoredat4°C. Onevialcontainsenoughmembranesforatleast200assays(units),whereanunitistheamountofmembranethatwillyieldgreaterthan5-foldsignal:backgroundwith125I-labeledIL-8at0.1nM |
| PhysicochemicalInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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