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当前位置: 首页 > 产品中心 > Functional_antibody > Millipore/MAB345 |抗O4抗体,克隆81/MAB345/50µ;G
商品详细Millipore/MAB345 |抗O4抗体,克隆81/MAB345/50µ;G
Millipore/MAB345 |抗O4抗体,克隆81/MAB345/50µ;G
Millipore/MAB345 |抗O4抗体,克隆81/MAB345/50µ;G
商品编号: MAB345
品牌: 密理博
市场价: ¥7400.00
美元价: 4440.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberMAB345
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-O4Antibody,clone81
AlternateNames
  • Sulfatide
BackgroundInformationOligodendrocytesandastrocytesarederivedfromcommonprecursorcells,glioblasts(Sommer,1981).O-Antigensaresulfatides,whichfunctionasdifferentiationMarkersonthesurfaceofoligodendrocytesofthecentralnervoussystem.O4isformedpostnatally(Schachner,1981)andisamarkerforcellbodiesandprocessesofoligodendrocytestypesIandII(hairyeyeballtype).Duringoligodendrocytedifferentiation,O4occursinpro-oligodendrocytes,butnotinO-2A-Progenitorcells.O4occursfromday3onwardsincellculturesofembryonicmousebrain.Anti-O4canbeusedinmyelination,demyelinationandremyelinationstudiesandinregenerationexperiments.
ProductInformation
FormatPurified
Control
  • Ratcorticalstemcellsorday3cellculturesofbrainsfrommouseembryos
PresentationPurifiedimmunoglobulinin0.05MPotassiumphosphatebuffer,pH8.0with0.3MNaCland0.05%sodiumazide.
StorageandShippingInformation
StorageConditionsMaintainfor1yearat2–8°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Applications
ApplicationAnti-O4Antibody,clone81isanantibodyagainstO4foruseinIC,IHwithmorethan50productcitations.
KeyApplications
  • Immunocytochemistry
  • Immunohistochemistry
ApplicationsNotRecommended
  • Immunoprecipitation
  • WesternBlotting
ApplicationNotesImmunohistochemistry:10-20μg/mLonunfixed,shockfrozentissue.

Immunocytochemistry:10-20μg/mLoncellsfixedwith4%paraformaldehyde.

Note:O4isasulfatide,whichcanbedissolvedoutofthemembranebyorganicsolvents;acetoneandmethanolshouldnotbeusedforfixation.

Optimalworkingdilutionsmustbedeterminedbytheenduser.

Immunohistochemistryprotocol

1.Preparesectionsfromunfixed,shockfrozentissue.Thesectionsshouldbe4-5μmthick.Placethesectionsonmicroscopeslides.

2.Washtheslidethreetimesfor5min.eachinPBSatroomtemperature.

3.Blockthenon-specificbindingsitesbyincubatingthesectionsinahumidchamberwith5%FCSatroomtemperaturefor30minutes.

4.Washtheslidesasdescribedinstep2.

5.CoverthesectionswithasufficientamountofMAB345(10-20μg/mLinPBS)andincubateinahumidchamberat37°Cforonehour.

6.WashtheslidesbrieflythreetimeswithPBS.Carefullydryaroundtheareatobestained.

7.Coverthesectionswithasufficientamountofanti-mouseIgM-fluorescein*solutionandincubateinahumidchamberat37°Cforonehour.

8.Washtheslidesasdescribedinstep6.

9.Coverthesectionswithasuitableembeddingmedium,coverwithacoverslip,andexaminebyfluorescencemicroscopy.



*HRPorABCcanalsobeused.



Optimalresultscanbeobtainedbytitratingtheprimaryandsecondaryantibodies



Immunocytochemistry



1.Fixthepreparationswith4%paraformaldehyde(inPBS)atroomtemperaturefor10minutes.O4isasulfatidewhichcanbedissolvedoutofthemembranebyorganicsolvents;acetoneandmethanolshouldnotbeusedforfixation.

2.Washtheslidethreetimesfor5min.eachinPBSatroomtemperature.

3.Blockthenon-specificbindingsitesbyincubatingthesectionsinahumanchamberwith5%FCSatroomtemperaturefor30minutes.

4.Washtheslidesasdescribedinstep2.

5.CoverthesectionswithasufficientamountofMAB345(10-20μg/mLinPBS)andincubateinahumidchamberat37°Cforonehour.

6.WashtheslidesbrieflythreetimeswithPBS.Carefullydryaroundtheareatobestained.

7.Coverthesectionswithasufficientamountofanti-mouseIgM-fluoresceinsolutionandincubateinahumidchamberat37°Cforonehour.

8.Washtheslidesasdescribedinstep6.

9.Coverthesectionswithasuitableembeddingmedium,coverwithacoverslip,andexaminebyfluorescencemicroscopy.



Note:Donotallowthepreparationstodryoutduringstaining.
BIOLOGicalInformation
ImmunogenHomogenateofwhitematterofcorpuscallosumfrombovinebrain.
Clone81(alsoreferredtointheliteratureasmABO4)
ConcentrationVaries,seelotspecificCoA
HostMouse
SpecificityRecognizesOligodendrocytemarkerO4.Alsoreactswithcertaingalactolipidsinsperm(seeAdditionalInformationlibraryforlist).
IsotypeIgM
SpeciesReactivity
  • Chicken
  • Human
  • Mouse
  • Rat
AntibodyTypeMonoclonalAntibody
PurificationMethodAmmoniumSulfatePrecipitation
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。