4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Millipore/AG400 | BLACK-GOLD®II染色剂/AG400/150 mg
商品详细Millipore/AG400 | BLACK-GOLD®II染色剂/AG400/150 mg
Millipore/AG400 | BLACK-GOLD®II染色剂/AG400/150 mg
Millipore/AG400 | BLACK-GOLD®II染色剂/AG400/150 mg
商品编号: AG400
品牌: 密理博
市场价: ¥7180.00
美元价: 4308.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberAG400
ReplacesAG390
TradeName
  • BLACK-GOLD
DescriptionBLACK-GOLD®IIStain
OverviewBlack-Gold®IIstainisanaurohalophosphatecomplexwhichstainsspecificallyformyelinwithinthecentralnervoussystem.Black-GoldIIstainingprovideshighresolution,highcontrast,ashorthistochemicalprocessingtime,andhighreproducibility.Black-GoldIIstainisanewandimprovedversionofitspredecessor,Black-Goldstain(discontinued).TheadvantagesofMillipore’sBlack-GoldIIoverBlack-GoldIstainarethatitismorereADIlysoluble,canbeutilizedatahigherconcentration,producesamoreuniformandconsistentstaining,takeslesstimetostain,anddoesnotrequirepost-stainingintensificationsteps.

TheuseofBlack-GoldIIstainistailoredtostudiesusingformalin-fixed,non-solventprocessedtissue.Thetechniquestainslargemyelinatedtractsdarkred-brown,whiletheindividualmyelinatedfibersappearblack.Thisnoveltracercanbeusedtolocalizebothnormalandpathologicalmyelin.Black-GoldIIstaincandemonstrateandcharacterizespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,methamphetamine,acrylamide,domoicacid,3-nitropropionicacid,Fluoro-Gold™tracerandisoniazid.

BlackGoldIIPurity:Nodetectableamountofuncomplexedgoldwasfound.

BlackGoldIIIllumination:Brightfieldordarkfield.

BlackGoldIISolubility:Freelysolubleinwater,saline,ordiluteacids.
ProductInformation
Components
  • Black-GoldII:150mgdrypowder,yellowincolor.
PresentationSeeComponents
StorageandShippingInformation
StorageConditionsThekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isusableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.
Applications
KeyApplications
  • Immunohistochemistry
ApplicationNotesTissueFixation:
Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue.

Sectioning:
Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.

Solutions:
Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

Staining:
1)Formalin-fixed,non-embeddedtissuesectionsarefirstmountedfromdistilledwaterontogelatin-coatedslidesandthenairdriedat50-60ºCforatleast½houronaslidewarmer.
2)TissuesectionsarethenrehydratedindistilledMilli-Q®waterforabout2minutes.
3)Theslidesarethentransferredtoa0.3%Black-GoldIIsolutiondissolvedin0.9%salinevehicleandheatedto60-65ºC.Afterabout12minutes,microscopicmonitoringoftheextentofthelabelingisrecommended.Thismonitoringshouldberepeatedevery2-3minutesuntilthedesireddegreeofmyelinimpregnationisobserved(seebelow).
4)Rinsetheslidesforabout2minindistilledMilli-Qwater.
5)Transfertheslidestoa1%sodiumthiosulfatesolutionfor3minutes.
6)Rinsetheslideswitheitherthree,fiveminchangesoftapwateror15minsofrunningtapwater.

Post-stainingsteps:
7)Dehydratesectionseitherviagraduatedalcoholicsolutionsorbyairdryingonaslidewarmer.
8)Immersesectionsfor1-2mininxyleneorhistoclearandthencoverslipwithanon-polarmountingmedia.
BIOLOGicalInformation
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。