密理博/AG105 |黑金II髓鞘染色试剂盒/AG105/1-免疫在线蚂蚁淘旗下平台

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商品详细密理博/AG105 |黑金II髓鞘染色试剂盒/AG105/1

密理博/AG105 |黑金II髓鞘染色试剂盒/AG105/1

商品编号： AG105

品牌：密理博

市场价： ¥8420.00

美元价： 5052.00

产地：美国(厂家直采)

公司：

产品分类：夹心法ELISA

公司分类： Sandwich_method_ELISA

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description
CatalogueNumber	AG105
TradeName	BLACK-GOLD
Description	BlackGoldIIMyelinStainingKit
Overview	Black-GoldIIisanaurohalophosphatecomplexwhichstainsspecificallyformyelinwithinthecentralnervoussystem.Black-GoldIIstainingprovideshighresolution,highcontrast,ashorthistochemicalprocessingtime,andhighreproducibility.Black-GoldIIisanewandimprovedversionofitspredecessor,Black-Gold(Cat#AG390).TheadvantagesofMillipore’sBlack-GoldIIMyelinStainingKitoverBlackGoldIarethatitismorereADIlysoluble,canbeutilizedatahigherconcentration,producesamoreuniformandconsistentstaining,takeslesstimetostain,anddoesnotrequirepost-stainingintensificationprocedures. TheuseofBlack-GoldIIistailoredtostudiesusingformalin-fixed,non-solventprocessedtissue.Thetechniquestainslargemyelinatedtractsdarkred-brown,whiletheindividualmyelinatedfibersappearblack.Thisnoveltracercanbeusedtolocalizebothnormalandpathologicalmyelin.Black-GoldIIcandemonstrateandcharacterizespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,methamphetamine,acrylamide,domoicacid,3-nitropropionicacid,Fluoro-Goldandisoniazid. TheBlack-GoldIIMyelinStainingKitalsocomeswithsodiumthiosulfateandcresylviolet.ThesodiumthiosulfatesolutionisrequiredforextractingexcessBlack-GoldIIstain.WhereasthecresylvioletisaNisslstainthatcolorscellbodiesabrilliantbluishhueandisusedasacounterstainwithBlack-GoldII. BlackGoldIIPurity:Nodetectableamountofuncomplexedgoldwasfound. BlackGoldIIIllumination:Brightfieldordarkfield. BlackGoldIISolubility:Freelysolubleinwater,saline,ordiluteacids.

ProductInformation
Components	Black-GoldII:150mgdrypowder,yellowincolor. CresylVioletStain:150mgdrypowder,purplish-blackincolor. 5%SodiumThiosulfate:10ml
Presentation	SeeComponents

StorageandShippingInformation
StorageConditions	Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isuseableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.

StorageandShippingInformation

StorageConditions

Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isuseableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.

Applications
KeyApplications	Immunohistochemistry
ApplicationNotes	TissueFixation: Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue. Sectioning: Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides. Solutions: Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. CresylVioletpowdershouldberesuspendedtoafinalconcentrationof0.1%usingamixtureofMilliQwaterandglacialaceticacid(3µlofacidpermlofwater).Onceresuspendedthecresylvioletsolutionshouldbestoredat4°Candisgoodforupto6months. Thesodiumthiosulfateshouldbedilutedto1%usingMilliQwaterpriortouse. Staining: Pre-heat0.3%Black-GoldIIand1%sodiumthiosulfatesolutionsto60°C.RehydratetissuesectionsinMilliQwaterfor2minutesthentransfertopre-warmedBlack-GoldIIsolution.IncubateslidesinBlack-GoldIIsolutionat60°Cfor12minutes.Atthispointtheslidesshouldbemonitoredat2-3minuteintervalstodeterminetheextentoflabeling.Stainingiscompletewhenthefinestmyelinatedfibersarestainingdarkredtoblack.Theappearanceofalavenderbackgroundcolorindicatesoverstainingandtheprocessshouldbestopped.Note:Theexactstainingtimewillvarydependingonsectionthickness,fixingconditions,typeoftissue,andageofstainsolution. Thesectionsarethenrinsed2XinMilliQwaterfor2minuteseach.Sodiumthiosulfatesolution(1%)isthenaddedandincubatedfor3minutesat60°C.Thesectionsarethenrinsed3XwithMilliQwaterfor2minuteseach.Cresylvioletsolutionisthenaddedandincubatedfor3minutesatroomtemperature.Thesectionsarethenrinsed3XwithMilliQwaterfor2minuteseach. Post-stainingsteps: Finally,dehydratetheslidesusingaseriesofgradatedalcohols.Thecresylstainwillleachfromthetissueatthistimeandtheextentofstainingcanbecontrolledbyhowlongtheseincubationslast.Thedehydratedsectionsarethenclearedinhistoclearorxylenefor2minutesandthencoverslippedwithmountingmedia. FrequentlyAskedQuestions(FAQs): 1)ItappearsthatMyelinimpregnationisincompletewithBlack-GoldII? Answer:Returnslidesto60°CBlack-GoldIIsolutionandmonitorevery2-3minutesundermicroscopeuntilfineparallelfibersoflayer1cortexarevisIBLe. 2)Backgroundhaslavendercolor? Answer:Sectionhasbeenoverstainedandstainingtimeshouldbereduced. 3)Backgroundstainingoccursbeforeimpregnationofallfinemyelinatedfibers? Answer:Tissuewasprobablyover-fixed.Trytoavoidleavingbraininfixative,orsectionsinbuffer,forlongerthanonemonth. 4)WhataretheadvantagesoverothermyelinstainslikeLuxolfastblue,orSudanBlack? Answer:Betterresolutionandcontrast.Faster. 5)DoothercounterstainsworkwithBlack-GoldII? Answer:Yes,mostconventionalNisslstains(includingblue,red,andgreen)canbeusedasacounterstain.Additionally,thecounterstaincanbeomittedifonlymyelinstainingisdesired.

Applications

KeyApplications

Immunohistochemistry

ApplicationNotes

TissueFixation:
Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue.

Sectioning:
Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.

Solutions:
Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.
CresylVioletpowdershouldberesuspendedtoafinalconcentrationof0.1%usingamixtureofMilliQwaterandglacialaceticacid(3µlofacidpermlofwater).Onceresuspendedthecresylvioletsolutionshouldbestoredat4°Candisgoodforupto6months.
Thesodiumthiosulfateshouldbedilutedto1%usingMilliQwaterpriortouse.

Staining:
Pre-heat0.3%Black-GoldIIand1%sodiumthiosulfatesolutionsto60°C.RehydratetissuesectionsinMilliQwaterfor2minutesthentransfertopre-warmedBlack-GoldIIsolution.IncubateslidesinBlack-GoldIIsolutionat60°Cfor12minutes.Atthispointtheslidesshouldbemonitoredat2-3minuteintervalstodeterminetheextentoflabeling.Stainingiscompletewhenthefinestmyelinatedfibersarestainingdarkredtoblack.Theappearanceofalavenderbackgroundcolorindicatesoverstainingandtheprocessshouldbestopped.Note:Theexactstainingtimewillvarydependingonsectionthickness,fixingconditions,typeoftissue,andageofstainsolution.

Thesectionsarethenrinsed2XinMilliQwaterfor2minuteseach.Sodiumthiosulfatesolution(1%)isthenaddedandincubatedfor3minutesat60°C.Thesectionsarethenrinsed3XwithMilliQwaterfor2minuteseach.Cresylvioletsolutionisthenaddedandincubatedfor3minutesatroomtemperature.Thesectionsarethenrinsed3XwithMilliQwaterfor2minuteseach.

Post-stainingsteps:
Finally,dehydratetheslidesusingaseriesofgradatedalcohols.Thecresylstainwillleachfromthetissueatthistimeandtheextentofstainingcanbecontrolledbyhowlongtheseincubationslast.Thedehydratedsectionsarethenclearedinhistoclearorxylenefor2minutesandthencoverslippedwithmountingmedia.

FrequentlyAskedQuestions(FAQs):

1)ItappearsthatMyelinimpregnationisincompletewithBlack-GoldII?
Answer:Returnslidesto60°CBlack-GoldIIsolutionandmonitorevery2-3minutesundermicroscopeuntilfineparallelfibersoflayer1cortexarevisIBLe.

2)Backgroundhaslavendercolor?
Answer:Sectionhasbeenoverstainedandstainingtimeshouldbereduced.

3)Backgroundstainingoccursbeforeimpregnationofallfinemyelinatedfibers?
Answer:Tissuewasprobablyover-fixed.Trytoavoidleavingbraininfixative,orsectionsinbuffer,forlongerthanonemonth.

4)WhataretheadvantagesoverothermyelinstainslikeLuxolfastblue,orSudanBlack?
Answer:Betterresolutionandcontrast.Faster.

5)DoothercounterstainsworkwithBlack-GoldII?
Answer:Yes,mostconventionalNisslstains(includingblue,red,andgreen)canbeusedasacounterstain.Additionally,thecounterstaincanbeomittedifonlymyelinstainingisdesired.

BIOLOGicalInformation

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

品牌介绍

密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。

公司介绍

品牌分类

细胞分析蛋白质样品制备细胞培养与系统蛋白质和酶抗体和化验抗体和化验蛋白质检测与定量生命科学研究抑制剂和生化物质

基因组分析批量和定制抗体药物发现与开发

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