密理博/AG105 |黑金II髓鞘染色试剂盒/AG105/1
市场价：
¥8420.00
美元价：
5052.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
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| CatalogueNumber | AG105 |
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| Description | BlackGoldIIMyelinStainingKit |
| Overview | Black-GoldIIisanaurohalophosphatecomplexwhichstainsspecificallyformyelinwithinthecentralnervoussystem.Black-GoldIIstainingprovideshighresolution,highcontrast,ashorthistochemicalprocessingtime,andhighreproducibility.Black-GoldIIisanewandimprovedversionofitspredecessor,Black-Gold(Cat#AG390).TheadvantagesofMillipore’sBlack-GoldIIMyelinStainingKitoverBlackGoldIarethatitismorereADIlysoluble,canbeutilizedatahigherconcentration,producesamoreuniformandconsistentstaining,takeslesstimetostain,anddoesnotrequirepost-stainingintensificationprocedures. TheuseofBlack-GoldIIistailoredtostudiesusingformalin-fixed,non-solventprocessedtissue.Thetechniquestainslargemyelinatedtractsdarkred-brown,whiletheindividualmyelinatedfibersappearblack.Thisnoveltracercanbeusedtolocalizebothnormalandpathologicalmyelin.Black-GoldIIcandemonstrateandcharacterizespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,methamphetamine,acrylamide,domoicacid,3-nitropropionicacid,Fluoro-Goldandisoniazid. TheBlack-GoldIIMyelinStainingKitalsocomeswithsodiumthiosulfateandcresylviolet.ThesodiumthiosulfatesolutionisrequiredforextractingexcessBlack-GoldIIstain.WhereasthecresylvioletisaNisslstainthatcolorscellbodiesabrilliantbluishhueandisusedasacounterstainwithBlack-GoldII. BlackGoldIIPurity:Nodetectableamountofuncomplexedgoldwasfound. BlackGoldIIIllumination:Brightfieldordarkfield. BlackGoldIISolubility:Freelysolubleinwater,saline,ordiluteacids. |
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| Presentation | SeeComponents |
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| StorageConditions | Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isuseableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption. |
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| ApplicationNotes | TissueFixation: Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue. Sectioning: Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides. Solutions: Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. CresylVioletpowdershouldberesuspendedtoafinalconcentrationof0.1%usingamixtureofMilliQwaterandglacialaceticacid(3µlofacidpermlofwater).Onceresuspendedthecresylvioletsolutionshouldbestoredat4°Candisgoodforupto6months. Thesodiumthiosulfateshouldbedilutedto1%usingMilliQwaterpriortouse. Staining: Pre-heat0.3%Black-GoldIIand1%sodiumthiosulfatesolutionsto60°C.RehydratetissuesectionsinMilliQwaterfor2minutesthentransfertopre-warmedBlack-GoldIIsolution.IncubateslidesinBlack-GoldIIsolutionat60°Cfor12minutes.Atthispointtheslidesshouldbemonitoredat2-3minuteintervalstodeterminetheextentoflabeling.Stainingiscompletewhenthefinestmyelinatedfibersarestainingdarkredtoblack.Theappearanceofalavenderbackgroundcolorindicatesoverstainingandtheprocessshouldbestopped.Note:Theexactstainingtimewillvarydependingonsectionthickness,fixingconditions,typeoftissue,andageofstainsolution. Thesectionsarethenrinsed2XinMilliQwaterfor2minuteseach.Sodiumthiosulfatesolution(1%)isthenaddedandincubatedfor3minutesat60°C.Thesectionsarethenrinsed3XwithMilliQwaterfor2minuteseach.Cresylvioletsolutionisthenaddedandincubatedfor3minutesatroomtemperature.Thesectionsarethenrinsed3XwithMilliQwaterfor2minuteseach. Post-stainingsteps: Finally,dehydratetheslidesusingaseriesofgradatedalcohols.Thecresylstainwillleachfromthetissueatthistimeandtheextentofstainingcanbecontrolledbyhowlongtheseincubationslast.Thedehydratedsectionsarethenclearedinhistoclearorxylenefor2minutesandthencoverslippedwithmountingmedia. FrequentlyAskedQuestions(FAQs): 1)ItappearsthatMyelinimpregnationisincompletewithBlack-GoldII? Answer:Returnslidesto60°CBlack-GoldIIsolutionandmonitorevery2-3minutesundermicroscopeuntilfineparallelfibersoflayer1cortexarevisIBLe. 2)Backgroundhaslavendercolor? Answer:Sectionhasbeenoverstainedandstainingtimeshouldbereduced. 3)Backgroundstainingoccursbeforeimpregnationofallfinemyelinatedfibers? Answer:Tissuewasprobablyover-fixed.Trytoavoidleavingbraininfixative,orsectionsinbuffer,forlongerthanonemonth. 4)WhataretheadvantagesoverothermyelinstainslikeLuxolfastblue,orSudanBlack? Answer:Betterresolutionandcontrast.Faster. 5)DoothercounterstainsworkwithBlack-GoldII? Answer:Yes,mostconventionalNisslstains(includingblue,red,andgreen)canbeusedasacounterstain.Additionally,thecounterstaincanbeomittedifonlymyelinstainingisdesired. |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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