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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Millipore/NS145 |多巴胺能神经元整合和表征试剂盒/NS145/1试剂盒
商品详细Millipore/NS145 |多巴胺能神经元整合和表征试剂盒/NS145/1试剂盒
Millipore/NS145 |多巴胺能神经元整合和表征试剂盒/NS145/1试剂盒
Millipore/NS145 |多巴胺能神经元整合和表征试剂盒/NS145/1试剂盒
商品编号: NS145
品牌: 密理博
市场价: ¥18460.00
美元价: 11076.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberNS145
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionDopaminergicNeuronIntegrationandCharacterizationKit
OverviewSeeindividualdatasheetsforspecificcomponentdescriptions.
BackgroundInformationDopaminergicneurondysfunctionandlosscorrelateswithnumerousprogressiveanddevastatingneurologicaldiseases.Stemcellresearchandapplicationsarerapidlymovingtowardtheintroductionandsubsequentpost-developmentanalysisofembryonicstem(ES)cell-deriveddopaminergicneuronsinadulttissue.TheNS145ispartofanexpandingChemicon/UpstateproductlinetargetingdevelopmentandplasticityinneuroBIOLOGy.NS145isanovel,broadlyencompassingkitforthelocalization,characterizationandanalysisofEScellderiveddopaminergicneuronintegrationintoadultcentralnervoussystem(CNS)tissue.Thiskitallowsresearchersto:(1)identifyandcharacterizedopaminergicneuronsintissue;and(2)identifyotherneighboringneuronaltypesandastrocytes.Inaddition,theantibodiesareamixtureofmouseandratmonoclonalsandrABItandgoatpolyclonalstooptimizedouble,triple,andquadruplestainingparADIgms.Thekitcomponentbreakdownisasfollows:

(1)Identifyingdopaminergicneurons.ResearchersareinterestedinidentifyingthesubtypeofneuronthattheirtaggedEShavematuredintofollowingintroductionandintegrationintotherecipientCNS.Thisisakeystepindopaminergicneuronreplacementtherapy.ThiskitincludesantibodiesfortheidentificationofdopaminergicneuronsusingseveraldiverseMarkerstargetingdifferentstagesintheneurotransmittersynthesisandmaintainance:DopaDecarboxylase(AB1569-50UL);TyrosineHydroxylase(TH;MAB5280-20UG);DopamineTransporter(DAT;MAB369-50UL).

(2)Identifyotherneuronalsubtypesandastrocytesneighboringthetransplantedcells.InordertomeasurethelevelofstructuralintegrationofthetaggedEScells,researchersoftenusegeneralmarkersofneuronalsubtypeandastrocytestocharacterizethelocalenvironmentinwhichtheEScell-deriveddopaminergicneuronsareintegrating.Thekitincludesantibodiesforthegeneralidentificationofneurons(NeuN;MAB377-100UG)andastrocytes(GFAP;AB5804).Alsoincludedareexcellentmarkersfortheidentificationofcholinergic(ChAT;AB144P-200UL)andSEROtonergic(Serotonin;MAB352-50UL)neurons.

Additional,individualreagentscanbeorderedseparatelyinlargerquantities.
ProductInformation
Components
  • Onekitcontainingthefollowingcomponents:
  • 100μgofNeuNmousemonoclonalMAB377-100UG
  • 50μLofDopaDecarboxylaserabbitpolyclonalAB1569-50UL
  • 50μLofDATratmonoclonalMAB369-50UL
  • 50μLofGFAPrabbitpolyclonalAB5804
  • 200μLofChATgoatpolyclonalAB144P-200UL
  • 50μLofSerotoninratmonoclonalMAB352-50UL
  • 20μgofTyrosineHydroxylasemousemonoclonalMAB5280-20UG
StorageandShippingInformation
StorageConditionsMaintaincomponentsaccordingtotheindividualdatasheets.

ImportantNote:Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatable-topcentrifugetodislodgeanyliquidinthecontainer"scap.
Applications
KeyApplications
  • Immunohistochemistry
  • Immunocytochemistry
BiologicalInformation
SpeciesReactivity
  • Vertebrates
EntrezGeneNumber
EntrezGeneSummaryThedopaminetransporter(DAT1)mediatestheactivereuptakeofdopaminefromthesynapseandisaprincipalregulatorofdopaminergicneurotransmission.TheDAT1genehasbeenimplicatedinhumandisorderssuchasparkinsonism,Tourettesyndrome,andsubstanceabuse(Vandenberghetal.,1992[PubMed1359373]).[suppliedbyOMIM]
GeneSymbol
  • DDC
  • AADC
  • EC4.1.1.28
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q01959#Aminetransporter.Terminatestheactionofdopaminebyitshighaffinitysodium-dependentreuptakeintopresynapticterminals.
SIZE:620aminoacids;68495Da
SUBUNIT:Homooligomer;disulfide-linked.InteractswithPRKCABPandTGFB1I1.
SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
SIMILARITY:SwissProt:Q01959##Belongstothesodium:neurotransmittersymporter(SNF)family.
MISCELLANEOUS:Thisproteinisthetargetofpsychomotorstimulantssuchasamphetaminesorcocaine.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。