当前位置：
首页
>
产品中心 >
Functional_antibody >
Millipore/MAB377B | Anti-NeuN Antibody, clone A60, biotin conjugated/MAB377B/500 µg
Millipore/MAB377B | Anti-NeuN Antibody, clone A60, biotin conjugated/MAB377B/500 µg
市场价：
¥7400.00
美元价：
4440.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MAB377B |
| BrandFamily | Chemicon® |
| TradeName |
|
| Description | Anti-NeuNAntibody,cloneA60,biotinconjugated |
| AlternateNames |
|
| BackgroundInformation | NeuNantibody(NEUronalNuclei;cloneA60)specificallyrecognizestheDNA-binding,neuron-specificproteinNeuN,whichispresentinmostCNSandPNSneuronalcelltypesofallvertebratestested.NeuNproteindistributionsareapparentlyrestrictedtoneuronalnuclei,perikaryaandsomeproximalneuronalprocessesinbothfetalandadultbrainalthough,someneuronsfailtoberecognizedbyNeuNatallages:INLretinalcells,Cajal-Retziuscells,Purkinjecells,inferiorolivaryanddentatenucleusneurons,andsympatheticganglioncellsareexamples(Mullenetal.,1992;Wolfetal.,1996).ImmunohistochemicallydetectableNeuNproteinfirstappearsatdevelopmentaltimepointsthatcorrespondwiththewithdrawaloftheneuronfromthecellcycleand/orwiththeinitiationofterminaldifferentiationoftheneuron(Mullenetal.,1992).ImmunoreactivityappearsaroundE9.5inthemouseneuraltubeandisextensivethroughoutthedevelopingnervoussystembyE12.5.Strongnuclearstainingsuggestsanuclearregulatoryproteinfunction;however,noevidencecurrentlyexistsastowhethertheNeuNproteinantigenhasafunctioninthedistalcytoplasmorwhetheritismerelysynthesizedtherebeforebeingtransportedbackintothenucleus.Nodifferencebetweenproteinisolatedfrompurifiednucleiandwholebrainextractonimmunoblotshasbeenfound(Mullenetal.,1992). |
| ProductInformation | |
|---|---|
| Format | Biotin |
| Control |
|
| Presentation | PurifiedmousemonoclonalIgG1inbuffercontaining0.01MPBSpH7.1,0.1%sodiumazidewith15mg/mLBSAasastABIlizer. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat2-8ºCfromdateofreceipt. |
| Applications | |
|---|---|
| Application | ThisAnti-NeuNAntibody,cloneA60,biotinconjugatedisvalidatedforuseinIC,IH,IH(P),WBforthedetectionofNeuN. |
| KeyApplications |
|
| ApplicationNotes | ImmunocytochemistryAnalysis: 1:10-1:500dilutionofapreviouslotwasused.Neuronsincultureshouldbepermeablizedwith0.1%tritonX-100.Allprimaryantibodydilutionsshouldbeperformedwithsimplesolutionscontainingonlybufferandprimaryantibodywithoutexcessproteinblocksordetergents. Forduallabelingstudiesusingmousemonoclonals,antibodyincubationsshouldbesequentialwithMAB377Blast.Firstmousemonoclonalantibodyshouldbefirstdetectedwithanti-mousesecondarypriortoincubatingwithMAB377B.Excessanti-mouseIgGmaybeblockedbyincubatingwith1%mouseserumpriortoMAB377Bincubation.DetectionofbiotinylatedNeuNmonoclonalisviastreptavidin.Insomecasesinmaybenecessarytopretreatthetissuewithavidintoblockexcessbiotinpriortoimmunohistochemisty(WoodandWarnke,1981). Immunohistochemistry: 1:200-1:2,000.Theantibodyworksbestonpolyesterwaxembeddedtissuebutalsoworksonparaffinembeddedtissueatalowerworkingdilution.Theantibodyworkswellwithformaldehyde-basedfixatives.Citricacidandmicrowavepretreatmenthasbeenusedsuccessfully(Sarnat,1998). WesternBlottingAnalysis: Apreviouslotofthisantibodywasusedonwesternblot.Recognizes2-3bandsinthe46-48kDarangeandpossIBLyanotherbandatapproximately66kDa. Optimalworkingdilutionsmustbedeterminedbyenduser. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Purifiedcellnucleifrommousebrain |
| Clone | A60 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | Vertebrateneuron-specificnuclearproteincalledNeuN(NeuronalNuclei).MAB377Breactswithmostneuronalcelltypesthroughoutthenervoussystemofmiceincludingcerebellum,cerebralcortex,hippocampus,thalamus,spinalcordandneuronsintheperipheralnervoussystemincludingdorsalrootganglia,sympatheticchaingangliaandentericganglia.Theimmunohistochemicalstainingisprimarilyinthenucleusoftheneuronswithlighterstaininginthecytoplasm.ThefewcelltypesnotreactivewithMAB377BincludePurkinje,mitralandphotoreceptorcells.Developmentally,immunoreactivityisfirstobservedshortlyafterneuronshavebecomepostmitotic,nostaininghasbeenobservedinproliferativezones.TheantibodyisanexcellentMarkerforneuronsinprimaryculturesandinretinoicacid-stimulatedP19cells.Itisalsousefulforidentifyingneuronsintransplants. |
| Isotype | IgG1 |
| SpeciesReactivity |
|
| SpeciesReactivityNote | Ratandmouse.Itisexpectedthatthebiotinconjugatedantibodywillalsoreactwithhuman,ferret,chickandsalamander. |
| AntibodyType | MonoclonalAntibody |
| GeneSymbol |
|
| PurificationMethod | ProteinAPurfied |
| MolecularWeight | 46-48kDa |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
