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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > 密理博/MAB2300X |密标™ 荧光泛神经标记物-Alexa488结合/MAB2300X/100µ;L
商品详细密理博/MAB2300X |密标™ 荧光泛神经标记物-Alexa488结合/MAB2300X/100µ;L
密理博/MAB2300X |密标™ 荧光泛神经标记物-Alexa488结合/MAB2300X/100µ;L
密理博/MAB2300X |密标™ 荧光泛神经标记物-Alexa488结合/MAB2300X/100µ;L
商品编号: MAB2300X
品牌: 密理博
市场价: ¥8420.00
美元价: 5052.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberMAB2300X
TradeName
  • Neuro-Chrom
DescriptionMilli-Mark™FluoroPanNeuronalMarker-Alexa488conjugated
BackgroundInformationAntibodiestoneuronalproteinshavebecomecriticaltoolsforidentifyingneuronsanddiscerningmorphologicalcharacteristicsincultureandcomplextissue.Whilethelabelingfromclassichistologicaltechniques,suchasGolgistaining,andmodernmolecularapproaches,suchasGFPconstructs,yieldexcellentcytoarchitecturaldetail,theseapproachesaretechnicallychallengingandimpracticalformanyneuroscienceresearchneeds.Neuron-specificantibodiesareconvenientprecisiontoolsusefulinrevealingcytoarchitecture,butarelimitedtotheproteintargetdistributionwithintheneuron,whichmaydiffergreatlyfromnucleustosomatodendriteandaxon.ToachieveascompleteamorphologicalstainingaspossIBLeacrossallpartsofneurons,Milliporepreviouslydevelopedamonoclonalantibodyblend(CatalogNo.MAB2300)thatreactsagainstkeysomatic,nuclear,dendritic,spine,andaxonalproteinsdistributedacrossthepan-neuronalarchitecture.NowMilliporehasformulatedMAB2300X,anAlexa488conjugatedversionofthepan-neuronalantibodyblendMAB2300tofacilitateimmunofluorescencestudies.EachantibodywithintheblendisconjugatedtoAlexa488thereforeenablingdetectionofanentireneuronwithonlyonemicroscopicemissionchannel.MAB2300Xhasbeenvalidatedindiversefixations,cellculture,andimmunohistochemistryprotocols,givingresearchersaconvenientandspecificqualitativeandquantitativetoolforstudyingneuronalmorphology.HighmorphologicalresolutionhasbeenachievedusingMAB2300xincludingdistraldentritesandspines.
ProductInformation
FormatAlexaFluor®488
Control
  • Corticalneurons
PresentationBlendedmonoclonalantibodycocktailinPBSwith0.05%NaN3
StorageandShippingInformation
StorageConditionsMaintainat2-8°Cforupto6monthsfromdateofreceipt.
Applications
ApplicationMilli-MarkFluoroPanNeuronalMarker-Alexa488conjugatedisanantibodytargetingtheFluoroPanNeuronalMarkerprotein,validatedforuseinICC&IF.
KeyApplications
  • Immunocytochemistry
  • Immunofluorescence
ApplicationNotesImmunocytochemistry:

1.Cultureneuronsasdesired.Whenready,gentlywashwithPBSandfixcellswith4%paraformaldehydeinPBS,30minatroomtemp(RT).
2.WashwithPBS,3x5mineach,atRTandblockwithblockingbuffer(1%BSA,5%Serum,0.2%TritonXinPBS)for1hratRT.
3.IncubatecellswithMilli-MarkFluoroPanNeuronalMarker(MouseIgGconjugatedwithAlexa488)for2hratRT(diluteantibodyinblockingbuffer).
4.WashwithPBS,3x5mineach,andcoverslipwithLIGHTDIAGNOSTICSMountingFluid(CatalogNo.#5013).ViewusingfluorescentmicroscopewithFITCfilter.
BIOLOGicalInformation
EpitopeWholeNeuronMarker
HostMouse
SpecificityMAB2300Xisspecifictoaxons(neurites),dendrites,spines,nucleus,andthecellbodyofneurons.
IsotypeIgG1
SpeciesReactivity
  • Human
  • Rat
  • Mouse
SpeciesReactivityNoteRat,Mouse,andHuman.Reactivitywithotherspecieshasnotbeendetermined.
AntibodyTypeMonoclonalAntibody
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。