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密理博/FCCS100182 | FlowCellect™ 组蛋白H2A.X磷酸化检测试剂盒/FCCS100182/100检测/；配套元件
密理博/FCCS100182 | FlowCellect™ 组蛋白H2A.X磷酸化检测试剂盒/FCCS100182/100检测/；配套元件
商品编号：
FCCS100182
品牌：
密理博
市场价：
¥10400.00
美元价：
6240.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | FCCS100182 |
| TradeName |
|
| Description | FlowCellect™HistoneH2A.XPhosphorylationAssayKit |
| Overview | Millipore’sFlowCellect™HistoneH2A.XPhosphorylationAssaykitincludesonedirectlyconjugatedantibody,aphospho-specificAnti-phospho-HistoneH2A.X(Ser139)-AlexaFluor®488conjugatedantibodytomeasurelevelsofHistoneH2A.X.ThiskitisdesignedtodetecttheextentofHistoneH2A.XpathwayactivationbymeasuringH2A.Xphosphorylationinanygivencellpopulation.ThekitprovidesasensitiveandvaluabletooltostudythefactorsthatinduceDNAdamageand/oraffectDNArepair,andallowonetoexplorethelinkagebetweenDNAdamage,cellcyclecheckpoints,andinitiationofapoptosis. IonizingrADIation(IR)andmanychemotherapeuticagentslikeetoposidekillcancercellsbyinductionofDNADSBs.Severalreportsshowthatthelevelofgamma-H2A.XasdetectedbyflowcytometrycorrelateswiththenumberofDNAstrandbreaks,tothelevelofcelldeathandradiosensitivity.H2A.XphosphorylatesinresponsetoaDNAdamagingreagent(e.g.Etoposide)orUVlight,anditsactivationclearlyindicatesthatDSBshaveoccurred.UnderstandingwhenDSBstakeplacecanhelpresearchersunderstandthemechanismsinvolvedinDNArepairandtheDNAdamageresponse. TheantibodyprovidedinthekithasbeencarefullytitratedtoensuretheABIlitytomeasurephospho-HistoneH2A.Xforaccuratedeterminationofproteinactivation.Sufficientreagentsareprovidedtoperform100tests.Detailedassayinstructionsareincludedtoassistinanalysisandtoensurethecorrectcellconcentrationisobtainedduringacquisitionofsampledata. |
| AlternateNames |
|
| BackgroundInformation | H2A.XisamemberofthehistoneH2Afamily.HistoneH2A.XresidesdownstreamoftheDNAdamagekinasesignalingcascade.PhosphorylationofHistoneH2A.Xatserine139isanimportantindicatorofDNAdamage.AsthelevelofDNAdamageincreases,thelevelofphosphoHistoneH2A.X(alsoknownasγH2AX)increases,accumulatingatthesitesofDNAdamage.ThisaccumulationofphosphoHistoneH2A.XisoftenusedtoindicatethelevelofDNAdamagepresentwithinthecell.H2AXisalsoresponsIBLeforrecruitingresponseproteinstothesiteofDNAdamageandmayplayaroleinDNArepair. Inall,acomprehensiveunderstandingofHistoneH2A.XactivityandunderstandingtheconsequenceofitsactivationcanprovidetheresearcherwithusefulinformationwhichwillbeimportantinunderstandingtheintrinsicnatureoftheDNAdamageresponse.Millipore’sFlowCellectHistoneH2A.XPhosphorylationAssayKitisdesignedtoallowtheresearchertomonitorandaccuratelymeasurephospho-specificHistoneH2A.Xactivationinapopulationofcells. AllFlowCellectkitsareoptimizedonguava®benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudytheHistoneH2A.Xsignalingpathwayrightinthecomfortoftheirownlab.Thedirectlyconjugatedantibodyprovidedinthekithasbeencarefullytitratedandoptimizedtoensuremaximalperformancealleviatingtheneedforanyadditionaloptimization.Thiskitcontainsoptimizedfixation,permeabilization,wash,andassaybufferstoprovideresearcherswithacompletesolutionforHistoneH2A.Xsignalinganalysis. |
| ProductInformation | |
|---|---|
| Components |
|
| Detectionmethod | Fluorescent |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 4-8°Cforantibodiesandbuffers |
| BIOLOGicalInformation | |
|---|---|
| Host | Mouse |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
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