当前位置：
首页
>
产品中心 >
Functional_antibody >
Millipore/MABE197 |抗磷酸视网膜母细胞瘤（Thr821）抗体，克隆E231，兔单克隆/MABE197/100µ；L
Millipore/MABE197 |抗磷酸视网膜母细胞瘤（Thr821）抗体，克隆E231，兔单克隆/MABE197/100µ；L
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | |
|---|---|
| CatalogueNumber | MABE197 |
| Description | Anti-phospho-Retinoblastoma(Thr821)Antibody,cloneE231,rabbitmonoclonal |
| AlternateNames |
|
| BackgroundInformation | Retinoblastomatumorsuppressorprotein(Rb)isassociatedwithDNAbindingactivityandisthoughttoplayasignificantroleincontrollingcellcycleprogressionduringtumorgrowth(1,2).Cellcycle-dependentphosphorylationbycdk’sinhibitsRbbinding,thusallowingcellcycleprogression(3).RbinactivationandcellcycleprogressionlikelyrequiresfirstphosphorylationbycyclinDcdk4/6followedbycyclinE-cdk2phosphorylation(4). |
| ProductInformation | |
|---|---|
| Format | DilutedCultureSupernatant |
| Control |
|
| Presentation | RabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor1yearat-20ºCfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow -20°CmaycauseglycerolcontainingSolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Syntheticphospho-peptidecorrespondingtohumanRbphosphorylatedatThr821. |
| Epitope | PhosphorylatedThr821 |
| Clone | E231 |
| Host | Rabbit |
| Isotype | IgG |
| SpeciesReactivity |
|
| AntibodyType | MonoclonalAntibody |
| GeneSymbol |
|
| Modifications |
|
| PurificationMethod | Unpurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.ActsasatranscriptionrepressorofE2F1targetgenes.Theunderphosphorylated,activeformofRB1interactswithE2F1andrepressesitstranscriptionactivity,leADIngtocellcyclearrest.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leadingtoepigenetictranscriptionalrepression.ControlshistoneH4"Lys-20"trimethylation.InhibitstheintrinsickinaseactivityofTAF1.MediatestranscriptionalrepressionbySMARCA4/BRG1byrecruitingahistonedeacetylase(HDAC)complextothec-FOSpromoter.Inrestingneurons,transcriptionofthec-FOSpromoterisinhibitedbyBRG1-dependentrecruitmentofaphospho-RB1-HDAC1repressorcomplex.Uponcalciuminflux,RB1isdephosphorylatedbycalcineurin,whichleadstoreleaseoftherepressorcomplexBysimilarity.Incaseofviralinfections,interactionswithSV40largeTantigen,HPVE7proteinoradenovirusE1AproteininducethedisassemblyofRB1-E2F1complextherebydisruptingRB1"sactivity. SUBUNITSTRUCTURE:InteractswithATAD5.InteractswithPRMT2Bysimilarity.ThehypophosphorylatedforminteractswithandsequesterstheE2F1transcriptionfactor.InteractswithheterodimericE2F/DPtranscriptionfactorcomplexescontainingTFDP1andeitherE2F1,E2F3,E2F4orE2F5,orTFDP2andE2F4.TheunphosphorylatedforminteractswithEID1,ARID3B,KDM5A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithNDC80.InteractswithGRIP1andUBR4.InteractswithARID4AandKDM5B.InteractswithE4F1andLIMD1.InteractswithSMARCA4/BRG1ANDHDAC1Bysimilarity.InteractswithadenovirusE1Aprotein,HPVE7proteinandSV40largeTantigen.InteractswithPSMA3andUSP4.Interacts(whenmethylatedatLys-860)withL3MBTL1. SUBCELLULARLOCATION:Nucleus. TISSUESPECIFICITY:Expressedintheretina. DOMAIN:ThePocketdomainbindstothethreonine-phosphorylateddomainC,therebypreventinginteractionwithheterodimericE2F/DPtranscriptionfactorcomplexes. PTM:PhosphorylatedinG1,therebyreleasingE2F1whichisthenabletoactivatecellgrowth.DephosphorylatedatthelateMphase.SV40largeTantigen,HPVE7andadenovirusE1Abindtotheunderphosphorylated,activeformofpRb.PhosphorylationatThr-821andThr-826promotesinteractionbetweentheC-terminaldomainCandthePocketdomain,andtherebyinhibitsinteractionswithheterodimericE2F/DPtranscriptionfactorcomplexes.DephosphorylatedatSer-795bycalcineruinuponcalciumstimulation. N-terminusismethylatedbyMETTL11A/NTM1Bysimilarity.MonomethylatedatLys-860bySMYD2,promotinginteractionwithL3MBTL1. INVOLVEMENTINDISEASE:DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20"000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil("cateye")isinvestigated. DefectsinRB1areacauseofsusceptibilitytobladdercancer(BLC)[MIM:109800].Amalignancyoriginatingintissuesoftheurinarybladder.Itoftenpresentswithmultipletumorsappearingatdifferenttimesandatdifferentsitesinthebladder.Mostbladdercancersaretransitionalcellcarcinomas.Theybeginincellsthatnormallymakeuptheinnerliningofthebladder.Othertypesofbladdercancerincludesquamouscellcarcinoma(cancerthatbeginsinthin,flatcells)andadenocarcinoma(cancerthatbeginsincellsthatmakeandreleasemucusandotherfluids).Bladdercancerisacomplexdisorderwithbothgeneticandenvironmentalinfluences. DefectsinRB1areacauseofosteogenicsarcoma(OSRC)[MIM:259500]. SEQUENCESIMILARITIES:Belongstotheretinoblastomaprotein(RB)family. |
| MolecularWeight | ~110observed |
| PhysicochemicalInformation |
|---|
| Dimensions |
|---|
| MaterialsInformation |
|---|
| MaterialsInformation |
|---|
品牌介绍
密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。
联络我们
