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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Millipore/ECM580 | QCM触觉细胞迁移分析-纤维连接蛋白,24孔,比色/ECM580/1 KIT足以用于
商品详细Millipore/ECM580 | QCM触觉细胞迁移分析-纤维连接蛋白,24孔,比色/ECM580/1 KIT足以用于
Millipore/ECM580 | QCM触觉细胞迁移分析-纤维连接蛋白,24孔,比色/ECM580/1 KIT足以用于
Millipore/ECM580 | QCM触觉细胞迁移分析-纤维连接蛋白,24孔,比色/ECM580/1 KIT足以用于
商品编号: ECM580
品牌: 密理博
市场价: ¥9540.00
美元价: 5724.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
CatalogueNumberECM580
ReplacesECM500
BrandFamilyChemicon®
TradeName
  • QCM
  • Chemicon
DescriptionQCMHaptotaxisCellMigrationAssay-Fibronectin,24-well,colorimetric
OverviewAlsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere

Introduction
TheCHEMICONQCM™HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricisidealforthestudyofepithelialandfibroblastcellhaptotaxis.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The8μmporesizeofthisassay"sBoydenchambersisnotappropriateforthestudyoflymphocytemigration.
•Eachkitprovidessufficientmaterialsfortheevaluationof12samples.
•Pleaserefertotheproductinsertforassaydetails.

TheCHEMICONQCM™HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications.
MaterialsRequiredbutNotDelivered1.PrecisionPipettes:sufficientforaliquotingcells.
2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Chemicon"sready-to-usenon-mammaliandetachmentsolution,Accutase™(Cat.No.SCR005)canalsobeused.
3.Tissueculturegrowthmediumappropriateforsubjectcells,suchasDMEMcontaining5%FBS.
4.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer.
5.SterilePBSorHBSStowashcells.
6.Distilledwater
7.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium.
8.Lowspeedcentrifugeandtubesforcellharvesting.
9.CO2incubatorappropriateforsubjectcells.
10.Hemocytometerorothermeansofcountingcells.
11.TrypanblueorequivalentviABIlitystain.
12.Microplatereader(540-570nmdetection)orspectrophotometer.
13.Sterilecellculturehood
14.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane.
15.Shaker
ProductInformation
Components
  • FibronectinTestPlate:(PartNo.2005689)One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
  • BSAControlPlate:(PartNo.2005791)One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
  • CellStainSolution*:(PartNo.20294)Onevial-10mL.
  • ExtractionBuffer:(PartNo.20295)Onevial-10mL.
  • 24wellStainExtractionPlate:(PartNo.2005871).Oneplate.
  • 96wellStainQuantitationPlate:(PartNo.2005870).Oneplate.
  • Swabs:(PartNo.10202)50each.
  • Forceps:(PartNo.10203)1pair
  • *CellStainSolutioncontainsasmallamountofcrystalviolet,whichistoxicifswallowedorinhaled,andmaycauseirritationtotheeyes,respiratorysystem,andskin.Handlewithcaution.
DetectionmethodChromogenic
StorageandShippingInformation
StorageConditionsStoreat4°Cforuptooneyearafterdateofpurchase.
Applications
ApplicationTheQCMHaptotaxisCellMigrationAssay,24-wellplate-Fibronectin,Colorimetricisidealforthestudyofepithelial&fibroblastcellhaptotaxis.
BIOLOGicalInformation
SpeciesReactivity
  • Vertebrates
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
品牌介绍
密理博(Millipore)公司成立于1954年,总部位于美国麻省,在全世界设有47个办事处,为100多个国家提供产品和技术服务。目前全球雇员超过5800人,在美国、法国和日本等国家拥有7家大型生产工厂,主要生产过滤膜及膜过滤产品。20世纪80年代,密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构,并于2000年4月在上海浦东外高桥保税区建立了密理博(上海)贸易有限公司。为了更好地满足中国用户的需求,密理博中国主页于2006年11月向广大用户开放,介绍密理博中国有限公司的最新动态,力求为用户打造专业的产品与服务信息交流平台。